Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Xue Chen; Yin Zhang; Yin Zhang; Yin Zhang; ShengQian Chao; ShengQian Chao; ShengQian Chao; LiLi Song; LiLi Song; LiLi Song; GuoGan Wu; GuoGan Wu; GuoGan Wu; Yu Sun; Yu Sun; Yu Sun; YiFan Chen; YiFan Chen; YiFan Chen; BeiBei Lv; BeiBei Lv; BeiBei Lv; BeiBei Lv; BeiBei Lv

doi:10.3389/fmicb.2024.1388669

Frontiers in Microbiology (May 2024)

Biocontrol potential of endophytic Bacillus subtilis A9 against rot disease of Morchella esculenta

Xue Chen,
Yin Zhang,
Yin Zhang,
Yin Zhang,
ShengQian Chao,
ShengQian Chao,
ShengQian Chao,
LiLi Song,
LiLi Song,
LiLi Song,
GuoGan Wu,
GuoGan Wu,
GuoGan Wu,
Yu Sun,
Yu Sun,
Yu Sun,
YiFan Chen,
YiFan Chen,
YiFan Chen,
BeiBei Lv,
BeiBei Lv,
BeiBei Lv,
BeiBei Lv,
BeiBei Lv

Affiliations

Xue Chen: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
Yin Zhang: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
Yin Zhang: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
Yin Zhang: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
ShengQian Chao: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
ShengQian Chao: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
ShengQian Chao: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
LiLi Song: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
LiLi Song: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
LiLi Song: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
GuoGan Wu: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
GuoGan Wu: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
GuoGan Wu: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
Yu Sun: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
Yu Sun: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
Yu Sun: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
YiFan Chen: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
YiFan Chen: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
YiFan Chen: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
BeiBei Lv: Biotechnology Research Institute, Key Laboratory of Agricultural Genetics and Breeding, Shanghai Academy of Agricultural Sciences, Shanghai, China
BeiBei Lv: Key Laboratory for Safety Assessment (Environment) of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, P.R, Shanghai, China
BeiBei Lv: Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing, Shanghai Academy of Agricultural Sciences, Shanghai, China
BeiBei Lv: Shanghai Co-Elite Agricultural Sci-Tech (Group) Co., Ltd., Shanghai, China
BeiBei Lv: CIMMYT-China Specialty Maize Research Center, Shanghai, China

DOI: https://doi.org/10.3389/fmicb.2024.1388669
Journal volume & issue: Vol. 15

Abstract

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IntroductionMorchella esculenta is a popular edible fungus with high economic and nutritional value. However, the rot disease caused by Lecanicillium aphanocladii, pose a serious threat to the quality and yield of M. esculenta. Biological control is one of the effective ways to control fungal diseases.Methods and resultsIn this study, an effective endophytic B. subtilis A9 for the control of M. esculenta rot disease was screened, and its biocontrol mechanism was studied by transcriptome analysis. In total, 122 strains of endophytic bacteria from M. esculenta, of which the antagonistic effect of Bacillus subtilis A9 on L. aphanocladii G1 reached 72.2% in vitro tests. Biological characteristics and genomic features of B. subtilis A9 were analyzed, and key antibiotic gene clusters were detected. Scanning electron microscope (SEM) observation showed that B. subtilis A9 affected the mycelium and spores of L. aphanocladii G1. In field experiments, the biological control effect of B. subtilis A9 reached to 62.5%. Furthermore, the transcritome profiling provides evidence of B. subtilis A9 bicontrol at the molecular level. A total of 1,246 differentially expressed genes (DEGs) were identified between the treatment and control group. Gene Ontology (GO) enrichment analysis showed that a large number of DEGs were related to antioxidant activity related. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the main pathways were Nitrogen metabolism, Pentose Phosphate Pathway (PPP) and Mitogen-Activated Protein Kinases (MAPK) signal pathway. Among them, some important genes such as carbonic anhydrase CA (H6S33_007248), catalase CAT (H6S33_001409), tRNA dihydrouridine synthase DusB (H6S33_001297) and NAD(P)-binding protein NAD(P) BP (H6S33_000823) were found. Furthermore, B. subtilis A9 considerably enhanced the M. esculenta activity of Polyphenol oxidase (POD), Superoxide dismutase (SOD), Phenylal anineammonia lyase (PAL) and Catalase (CAT).ConclusionThis study presents the innovative utilization of B. subtilis A9, for effectively controlling M. esculenta rot disease. This will lay a foundation for biological control in Morchella, which may lead to the improvement of new biocontrol agents for production.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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