Clinical, Cosmetic and Investigational Dermatology (Mar 2021)

Comparative Analysis of Hyaluronidase-Mediated Degradation Among Seven Hyaluronic Acid Fillers in Hairless Mice

  • Kwak SS,
  • Yoon KH,
  • Kwon JH,
  • Kang WH,
  • Rhee CH,
  • Yang GH,
  • Cruz DJM,
  • Son WC

Journal volume & issue
Vol. Volume 14
pp. 241 – 248

Abstract

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Seong Sung Kwak,1,2 Kwang Ho Yoon,1 Jin Hee Kwon,1 Won-Ho Kang,1 Chang-Hoon Rhee,3 Gi-Hyeok Yang,1 Deu John M Cruz,1 Woo-Chan Son2 1Gwanggyo R&D Center, Medytox Inc., Suwon-si, Gyeonggi-do, Korea; 2Department of Pathology, Asan Medical Center, Seoul, Korea; 3Osong R&D Center, Medytox Inc., Cheongju-si, Chungcheongbuk-do, KoreaCorrespondence: Woo-Chan SonDepartment of Pathology, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-Gil, Songpa-gu, Seoul, Republic of KoreaTel +82 2 3010 4051Fax +82 2 3010 4099Email [email protected] John M CruzNon-Clinical Development Department, Medytox Gwanggyo R&D Center, 114 Central town-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of KoreaTel +82 31 8065 8254Fax +82 31 8065 8232Email [email protected]: Hyaluronic acid (HA) is the most common injectable dermal filler used for soft-tissue augmentation, and can be removed non-surgically by directly injecting hyaluronidase. In this study, the hyaluronidase-mediated degradation of different types of HA fillers implanted subcutaneously at the back of hairless mice having filler residence time of four days or three months were compared.Methods: Two sites at the back of female hairless mice were subcutaneously implanted with 0.1-mL of one of the seven HA fillers (NLL, NL, NDL, NVL, and ND, JUVX+, and RESLYFT) and injected with 30 IU or 60 IU hyaluronidase per 0.1-mL filler after reaching a filler residence time of 4 or 91 days, respectively. Filler bolus projection was measured using three-dimensional optical imaging over a 72 h period, and the implantation sites were histologically examined 2 weeks after hyaluronidase injection.Results: Following hyaluronidase injection, all seven HA fillers showed a rapid decrease of filler volume within 24 h, and complete degradation was confirmed by histological examination after 2 weeks. There was no significant difference in filler volume reduction rate among the seven HA fillers, and no evidence of macroscopic or microscopic adverse effects were observed at the implantation sites.Conclusion: All seven HA fillers show comparable susceptibility to hyaluronidase-mediated degradation. HA fillers with prolonged filler residence time may require a higher dose of hyaluronidase to achieve efficient degradation owing to tissue integration.Keywords: soft tissue augmentation, HA filler volume, 3D optical imaging, filler residence time, tissue integration

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