GluLa-DPG-PEG600 nanopolymer binds proteins and spreads to rats’ organs and tissues

Abstract

In this article, we described the ability of GluLa-DPG-PEG600 nanopolymer based on the pseudopolyamino acids to bind proteins and its localization in rats organs and tissues after intravenous and intramuscular injections. By using electrophoresis in 5% polyacrylamide gel, it was found the ability of GluLa-DPG-PEG600 nanopolymer to bind bovine serum albumin (BSA). This indicates the GluLa-DPG-PEG600 is a potential transporter of proteins and their complexes. By means of fluorescent microscopy, it was found that GluLa-DPG-PEG600 nanopolymer labeled with fluorescein with BSA labeled with fluorescent Alexa Fluor 555 dye (GluLa-DPG-PEG600-F + BSA Alexa Fluor 555) localized in liver and brain on the 16th after intramuscular injection. On the 5th hour after intravenous injection, GluLa-DPG-PEG600-F + BSA Alexa Fluor 555 complex localized in spleen and kidney obtained. Results show a potential usefulness of GluLa-DPG-PEG600 as a transporter of drugs capable of penetrating the blood-brain barrier. Locali­zation of GluLa-DPG-PEG600-F + BSA Alexa Fluor 555 complex in spleen suggests that GluLa-DPG-PEG600 could be used as an adjuvant for development of vaccines.

Keywords

• rats
• nanopolymer
• pseudopolyamino acids
• drug transporter
• adjuvant