Insects (Dec 2021)

Standard Methods for Dissection of <i>Varroa destructor</i> Females

  • Vincent Piou,
  • Caroline Vilarem,
  • Carolin Rein,
  • Lina Sprau,
  • Angélique Vétillard

DOI
https://doi.org/10.3390/insects13010037
Journal volume & issue
Vol. 13, no. 1
p. 37

Abstract

Read online

Varroa destructor (Anderson and Trueman) is known as a major pest of Apis mellifera L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle. Studies focusing on isolated organs are needed to better comprehend this organism. To conduct such targeted molecular or physiological studies, the dissection of V. destructor mites is crucial as it allows the extraction of specific organs. Here, we propose a technical article showing detailed steps of females V. destructor dissection, illustrated with pictures and videos. These illustrated guidelines will represent a helpful tool to go further in V. destructor research.

Keywords