Standard Methods for Dissection of <i>Varroa destructor</i> Females
Vincent Piou,
Caroline Vilarem,
Carolin Rein,
Lina Sprau,
Angélique Vétillard
Affiliations
Vincent Piou
Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France
Caroline Vilarem
Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France
Carolin Rein
Apicultural State Institute, University of Hohenheim, Erna-Hruschka-Weg 6, 70599 Stuttgart, Germany
Lina Sprau
Department of Livestock Population Genomics, University of Hohenheim, Garbenstr. 17, 70599 Stuttgart, Germany
Angélique Vétillard
Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France
Varroa destructor (Anderson and Trueman) is known as a major pest of Apis mellifera L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle. Studies focusing on isolated organs are needed to better comprehend this organism. To conduct such targeted molecular or physiological studies, the dissection of V. destructor mites is crucial as it allows the extraction of specific organs. Here, we propose a technical article showing detailed steps of females V. destructor dissection, illustrated with pictures and videos. These illustrated guidelines will represent a helpful tool to go further in V. destructor research.