Терапевтический архив (Oct 2012)
Cytokine gene polymorphism in type 2 diabetes mellitus in the Europeoid women of Russia
Abstract
Aim. To study the distribution of genotypes in the cytokine genes and their combinations with immunoregulatory activity in patients with type 2 diabetes mellitus (T2DM) and in healthy women. Subjects and methods. 586 Europeoid women from the eastern regions of Russia, including 374 healthy women aged 23-68 years and 212 women aged 28-69 years with T2DM complicated and uncomplicated by osteoporosis, were examined. Seven polymorphisms located in the promoter regions of the interleukin (IL) gene: TNF-α at positions С-863А, G-308A, G-238A, IL1B T-31C, IL6 G-174C, IL10 C-592A, VEGFA С-2578А were investigated. Restriction analysis of amplification products was applied. Results. There were high associations of the predisposition and resistance to the development of T2DM with a number of polylocus cytokine genotype combinations having pro- and anti-inflammatory, angiogenic, and immunoregulatory activities. The association of the cytokine genes with T2DM was found to be mediated in nature through a relationship of the genotypes to the high or low production of regulatory cytokines and to different factors of regulation of lipid and carbohydrate metabolisms, inflammation, and bone remodeling. Conclusion. The high odds ratio and high specificity of the detected genetic combinations allow one to hope that they will be clinically used as predictors.
