Frontiers in Cell and Developmental Biology (Nov 2021)

CRISPR/Cas System and Factors Affecting Its Precision and Efficiency

  • Nasir Javaid,
  • Sangdun Choi,
  • Sangdun Choi

DOI
https://doi.org/10.3389/fcell.2021.761709
Journal volume & issue
Vol. 9

Abstract

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The diverse applications of genetically modified cells and organisms require more precise and efficient genome-editing tool such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas). The CRISPR/Cas system was originally discovered in bacteria as a part of adaptive-immune system with multiple types. Its engineered versions involve multiple host DNA-repair pathways in order to perform genome editing in host cells. However, it is still challenging to get maximum genome-editing efficiency with fewer or no off-targets. Here, we focused on factors affecting the genome-editing efficiency and precision of CRISPR/Cas system along with its defense-mechanism, orthologues, and applications.

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