Interaction of TiO2 nanoparticles with the C. vulgaris: oxidative stress, lipid peroxidation and lipid amount

Abstract

Nanoparticles are widely used in many industries such as food and cosmetics. With increasing use, its spread to environmental environments is also increasing. Microalgae have an important place in the uptake of nanoparticles into the food chain. In this study, the effect of TiO2 nanoparticle on antioxidant enzyme activity, malondialdehyde, hydrogen peroxide, chlorophyll-a and total lipid amount in C. vulgaris microalgae has been investigated. As a result of the dose study, while the superoxide dismutase and ascorbate peroxidase enzyme activities decreased, the amount of MDA, H2O2 and chlorophyll-a increased. Depending on the times at different light:dark ratios, both an increase and a decrease occurred in the SOD, APX enzyme activity and the amount of MDA, H2O2. There was an increase in the amount of chlorophyll-a. In the time study, while the SOD and APX enzyme activities increased, the amount of MDA and H2O2 decreased. The amount of chlorophyll-a increased. In the total lipid study, the total lipid amount in the group with nano TiO2 increased compared to the control group. At the same time, C18:2 T (linoleic acid) has been found as fatty acid methyl ester in both groups. HIGHLIGHTS The effect of n.TiO2 on SOD, APX, LPO, H2O2 has been investigated in C.vulgaris.; The effect of n.TiO2 on chlorophyll-a, lipid and FAME have been investigated for C.vulgaris.; The most affected n.TiO2 dose was 5 & 100 mg/L and the exposure time was 12 and 96 h.; The most affected the light-dark ratio was 18L:6D and 6L:18D for C.vulgaris.; Total lipid content increased in the presence of n.TiO2.;

Keywords

• apx
• chlorophyll-a
• h2o2
• lipid
• mda
• n.tio2
• sod