Acta Pharmaceutica Sinica B (Jul 2018)

Biocatalytic access to diverse prenylflavonoids by combining a regiospecific C-prenyltransferase and a stereospecific chalcone isomerase

  • Jianhua Li,
  • Ridao Chen,
  • Ruishan Wang,
  • Xiao Liu,
  • Kebo Xie,
  • Dawei Chen,
  • Jungui Dai

DOI
https://doi.org/10.1016/j.apsb.2018.01.009
Journal volume & issue
Vol. 8, no. 4
pp. 678 – 686

Abstract

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Prenylflavonoids are valuable natural products that have diverse biological properties, and are usually generated biologically by multiple metabolic enzymes in nature. In this study, structurally diverse prenylflavonoids were conveniently synthesized by enzymatic catalysis by combining GuILDT, a regiospecific chalcone prenyltransferase, and GuCHI, a stereospecific chalcone isomerase that has promiscuous activity for both chalcones and prenylchalcones as substrates. Our findings provided a new approach for the synthesis of natural/unnatural bioactive prenylflavonoids, including prenylchalcones and optical prenylflavanones with chalcone origins. KEY WORDS: Biocatalysis, Prenyltransferase, Chalcone isomerase, Licorice, Prenylflavonoids