An in vitro evaluation of antibacterial properties of 2 pulp capping materials

Abstract

Objective To evaluate the antimicrobial properties of 2 different pulp capping materials (ProRoot MTA and iRoot BP Plus).Methods Modified direct contact test (DCT) was performed as follow. ProRoot MTA and iRoot BP Plus were set for 7 days, then crushed and immersed into deionized water at concentrations of 200 g·L -1 for another 7 days. PH values of the supernatants of the aqueous extracts were measured with a pH meter. The germfree supernatants were mixed with Streptococcus mutans suspensions and then incubated for 24 hours. The survival of bacteria was assessed by measuring the optical density (OD) at 600 nm with an automated microplate reader.Results PH values of the aqueous extracts of ProRoot MTA and iRoot BP Plus were between 12 and 13, and no statistically significant difference was found (t = 0.133, P = 0.899). Both ProRoot MTA and iRoot BP Plus demonstrated good antibacterial properties (P < 0.05), and iRoot BP Plus performed a better antibacterial effect (P < 0.05).Conclusion IRoot BP Plus performed a better antibacterial effect against Streptococcus mutans when compared to ProRoot MTA.

Keywords

• Antibacterial property，
• ProRoot MTA，
• iRoot BP Plus，
• Streptococcus mutans ，
• Direct contact test，