Generation of a human iPSC line harboring a biallelic large deletion at the INK4 locus (HMGUi001-A-5)

Alireza Shahryari; Noel Moya; Johanna Siehler; Xianming Wang; Anna Karolina Blöchinger; Ingo Burtscher; Mostafa Bakhti; Seyed Javad Mowla; Heiko Lickert

Stem Cell Research (Aug 2020)

Generation of a human iPSC line harboring a biallelic large deletion at the INK4 locus (HMGUi001-A-5)

Alireza Shahryari,
Noel Moya,
Johanna Siehler,
Xianming Wang,
Anna Karolina Blöchinger,
Ingo Burtscher,
Mostafa Bakhti,
Seyed Javad Mowla,
Heiko Lickert

Affiliations

Alireza Shahryari: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Technical University of Munich, School of Medicine, Department of Human Genetics, Klinikum Rechts der Isar, 81675 München, Germany; Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Noel Moya: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany
Johanna Siehler: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Technical University of Munich, School of Medicine, Department of Human Genetics, Klinikum Rechts der Isar, 81675 München, Germany
Xianming Wang: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Technical University of Munich, School of Medicine, Department of Human Genetics, Klinikum Rechts der Isar, 81675 München, Germany
Anna Karolina Blöchinger: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Technical University of Munich, School of Medicine, Department of Human Genetics, Klinikum Rechts der Isar, 81675 München, Germany
Ingo Burtscher: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany
Mostafa Bakhti: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany
Seyed Javad Mowla: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Corresponding authors at: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran (S.J. Mowla); Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany (H. Lickert).
Heiko Lickert: Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Institute of Stem Cell Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany; Technical University of Munich, School of Medicine, Department of Human Genetics, Klinikum Rechts der Isar, 81675 München, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany; Corresponding authors at: Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran (S.J. Mowla); Institute of Diabetes and Regeneration Research, Helmholtz Zentrum München, D-85764 Neuherberg, Germany (H. Lickert).

Journal volume & issue: Vol. 47
p. 101927

Abstract

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The INK4 locus is considered as a hot-spot region for the complex genetic disorders, including cancer, type 2 diabetes (T2D) and coronary artery disease (CAD). By CRISPR/Cas9 gene editing, we generated a human induced pluripotent stem cell (hiPSC) line (HMGUi001-A-5) deleting an 8 kb genomic DNA encompassing six T2D-associated SNPs at the INK4 locus. The resulting hiPSC line revealed a normal karyotype, preserved pluripotency and was able to differentiate towards germ layers, endoderm, mesoderm and ectoderm. Thus, the HMGUi001-A-5 line could provide a valuable cellular model to explore the molecular mechanisms linking these SNPs to T2D and other genetic disorders.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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