Frontiers in Genome Editing (Nov 2021)

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

  • Jonas Holst Wolff,
  • Jakob Haldrup,
  • Emil Aagaard Thomsen,
  • Sofie Andersen,
  • Jacob Giehm Mikkelsen

DOI
https://doi.org/10.3389/fgeed.2021.786893
Journal volume & issue
Vol. 3

Abstract

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Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.

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