An effective strategy for preparation of a polyclonal antibody with an addition of carbon chain of ciprofloxacin

Abstract

In this study, we synthesized amino propyl ciprofloxacin (CPLX-NH 2 ) as a ciprofloxacin (CPLX) derivative. Moreover, the immune antigen CPLX-NH 2 -BSA and coating antigen CPLX-NH 2 -OVA were prepared via CPLX-NH 2 coupling with bovine serum albumin (BSA) and ovalbumin (OVA), respectively. Subsequently, the Kunming mice were immunized with immune antigen to obtain the polyclonal antibody with high titer. The regression equation of CPLX-NH 2 antibody was y = –17.395x + 89.331 (R 2 = 0.9961); IC 50 and limit of detection (LOD) were 182.39 and 20.09 ng/mL, respectively. These results were superior to that of CPLX antibody. Meanwhile, the CPLX-NH 2 antibody showed cross-reactivity to fluoroquinolones (FQNs) residues. The results of the study indicated that the proper modification of the drug, namely, the addition of a suitable spacer arm between the drug and the carrier protein will improve the efficacy of the antibody, which is a favorable concept for preparation of antibody.