Frontiers in Microbiology (Feb 2022)
Respiratory Mucosal Immunity: Kinetics of Secretory Immunoglobulin A in Sputum and Throat Swabs From COVID-19 Patients and Vaccine Recipients
- Cuiping Ren,
- Cuiping Ren,
- Cuiping Ren,
- Cuiping Ren,
- Cuiping Ren,
- Yong Gao,
- Cong Zhang,
- Cong Zhang,
- Cong Zhang,
- Cong Zhang,
- Cong Zhang,
- Chang Zhou,
- Chang Zhou,
- Chang Zhou,
- Chang Zhou,
- Chang Zhou,
- Ying Hong,
- Mingsheng Qu,
- Mingsheng Qu,
- Mingsheng Qu,
- Mingsheng Qu,
- Mingsheng Qu,
- Zhirong Zhao,
- Yinan Du,
- Yinan Du,
- Yinan Du,
- Yinan Du,
- Yinan Du,
- Li Yang,
- Li Yang,
- Li Yang,
- Li Yang,
- Li Yang,
- Boyu Liu,
- Boyu Liu,
- Boyu Liu,
- Boyu Liu,
- Boyu Liu,
- Siying Wang,
- Siying Wang,
- Siying Wang,
- Siying Wang,
- Siying Wang,
- Mingfeng Han,
- Yuxian Shen,
- Yuxian Shen,
- Yuxian Shen,
- Yuxian Shen,
- Yuxian Shen,
- Yan Liu,
- Yan Liu,
- Yan Liu,
- Yan Liu,
- Yan Liu
Affiliations
- Cuiping Ren
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Cuiping Ren
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Cuiping Ren
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Cuiping Ren
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Cuiping Ren
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Yong Gao
- Department of Clinical Laboratory, The Second People’s Hospital of Fuyang, Fuyang, China
- Cong Zhang
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Cong Zhang
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Cong Zhang
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Cong Zhang
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Cong Zhang
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Chang Zhou
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Chang Zhou
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Chang Zhou
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Chang Zhou
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Chang Zhou
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Ying Hong
- Maanshan Center for Disease Control and Prevention, Maanshan, China
- Mingsheng Qu
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Mingsheng Qu
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Mingsheng Qu
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Mingsheng Qu
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Mingsheng Qu
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Zhirong Zhao
- Maanshan Center for Disease Control and Prevention, Maanshan, China
- Yinan Du
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Yinan Du
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Yinan Du
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Yinan Du
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Yinan Du
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Li Yang
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Li Yang
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Li Yang
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Li Yang
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Li Yang
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Boyu Liu
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Boyu Liu
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Boyu Liu
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Boyu Liu
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Boyu Liu
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Siying Wang
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Siying Wang
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Siying Wang
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Siying Wang
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Siying Wang
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Mingfeng Han
- Department of Clinical Laboratory, The Second People’s Hospital of Fuyang, Fuyang, China
- Yuxian Shen
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Yuxian Shen
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Yuxian Shen
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Yuxian Shen
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Yuxian Shen
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- Yan Liu
- Department of Microbiology and Parasitology, Anhui Medical University, Hefei, China
- Yan Liu
- Anhui Provincial Laboratory of Pathogen Biology, Anhui Medical University, Hefei, China
- Yan Liu
- Anhui Key Laboratory of Zoonosis of High Institution, Anhui Medical University, Hefei, China
- Yan Liu
- Laboratory of Tropical and Parasitic Diseases Control, Anhui Medical University, Hefei, China
- Yan Liu
- School of Basic Medical Sciences, Anhui Medical University, Hefei, China
- DOI
- https://doi.org/10.3389/fmicb.2022.782421
- Journal volume & issue
-
Vol. 13
Abstract
While IgM and IgG response to SARS-CoV-2 has been extensively studied, relatively little is known about secretory IgA (sIgA) response in respiratory mucosa. Here we report IgA response to the SARS-CoV-2 in sputum, throat swabs, and serum with nucleocapsid protein (NP) enzyme-linked immunosorbent assays (ELISA) in a cohort of 28 COVID-19 patients and 55 vaccine recipients. The assays showed sIgA in respiratory mucosa could be detected on the first day after illness onset (AIO), and the median conversion time for sIgA in sputum, throat swabs, and serum was 3, 4, and 10 days, respectively. The positive rates of sIgA first week AIO were 100% (24/28) and 85.7% (24/28) in sputum and throat swabs, respectively, and were both 100% during the mid-onset (2–3 weeks AIO). During the recovery period, sIgA positive rates in sputum and throat swabs gradually decreased from 60.7% (17/28) and 57.1% (16/28) 1 month AIO and the sIgA antibodies were all undetectable 6 months AIO. However, serum IgA positive rate was still 100% at 4 months and 53.6% (15/28) at 6 months. Throat swabs obtained from volunteers who received inactivated SARS-CoV-2 vaccines by intramuscular delivery all showed negative results in IgA ELISA. These findings will likely improve our understanding of respiratory mucosal immunity of this emerging disease and help in containing the pandemic and developing vaccines.
Keywords
