Chinese Journal of Lung Cancer (Dec 2010)

Expressions and Significances of PRL-3 and RhoC in A549 Cell

  • Ping ZHANG,
  • Zhipei ZHANG,
  • Xiangmin LI,
  • Jie LEI,
  • Kai SU,
  • Xiaofei LI,
  • Xiaoping WANG

Journal volume & issue
Vol. 13, no. 12
pp. 1160 – 1164

Abstract

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Background and objective The expression of phosphatase of regenerating liver-3 (PRL-3) is correlated with Ras homologue C (RhoC) in non-small cell lung cancer (NSCLC), suggesting that they have interactions. The aim of this study is to investigate the functions of PRL-3 and RhoC in the migration of A549 cell and the potential mechanism of PRL-3 and RhoC in carcinogenesis and cancer development. Methods PRL-3Ab and RhoCAb were used to block the functions of PRL-3 and RhoC respectively. Wound healing assay was applied to detect the migration of A549 cell and the expression levels of PRL-3 and RhoC were detected by RT-PCR. Results The migration of A549 cell decreased after blockage of PRL-3 and RhoC. The expression of RhoC decreased when PRL-3 was blocked without any changes on the expression of PRL-3. Conclusion PRL-3, RhoC could increase cell migration in A549 cells.

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