Chinese Journal of Lung Cancer (Dec 2010)
Expressions and Significances of PRL-3 and RhoC in A549 Cell
Abstract
Background and objective The expression of phosphatase of regenerating liver-3 (PRL-3) is correlated with Ras homologue C (RhoC) in non-small cell lung cancer (NSCLC), suggesting that they have interactions. The aim of this study is to investigate the functions of PRL-3 and RhoC in the migration of A549 cell and the potential mechanism of PRL-3 and RhoC in carcinogenesis and cancer development. Methods PRL-3Ab and RhoCAb were used to block the functions of PRL-3 and RhoC respectively. Wound healing assay was applied to detect the migration of A549 cell and the expression levels of PRL-3 and RhoC were detected by RT-PCR. Results The migration of A549 cell decreased after blockage of PRL-3 and RhoC. The expression of RhoC decreased when PRL-3 was blocked without any changes on the expression of PRL-3. Conclusion PRL-3, RhoC could increase cell migration in A549 cells.