Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats

Sanbao Zhang; Qiongwen Zhang; Lili Yang; Xiaotong Gao; Ting Chen; Tianbao Li; Wenyue Sun; Yufan Liu; Zihua Zheng; Yan Pan; Yingming Wei; Yanna Huang; Mingsheng Jiang; Qinyang Jiang

doi:10.3390/genes14020315

Genes (Jan 2023)

Comparative and Functional Analysis of miRNAs and mRNAs Involved in Muscle Fiber Hypertrophy of Juvenile and Adult Goats

Sanbao Zhang,
Qiongwen Zhang,
Lili Yang,
Xiaotong Gao,
Ting Chen,
Tianbao Li,
Wenyue Sun,
Yufan Liu,
Zihua Zheng,
Yan Pan,
Yingming Wei,
Yanna Huang,
Mingsheng Jiang,
Qinyang Jiang

Affiliations

Sanbao Zhang: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Qiongwen Zhang: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Lili Yang: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Xiaotong Gao: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Ting Chen: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Tianbao Li: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Wenyue Sun: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Yufan Liu: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Zihua Zheng: Institute for New Rural Development, Guangxi University, Nanning 530004, China
Yan Pan: College of Animal Science and Technology, Guangxi Agricultural Vocational University, Nanning 530007, China
Yingming Wei: Institute for New Rural Development, Guangxi University, Nanning 530004, China
Yanna Huang: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Mingsheng Jiang: College of Animal Science and Technology, Guangxi University, Nanning 530004, China
Qinyang Jiang: College of Animal Science and Technology, Guangxi University, Nanning 530004, China

DOI: https://doi.org/10.3390/genes14020315
Journal volume & issue: Vol. 14, no. 2
p. 315

Abstract

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MicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate several pathway intermediates and affect the skeletal muscle development in mice, pigs, sheep, and cattle. However, to date, only a small number of miRNAs have been reported in the muscle development of goats. In this report, the longissimus dorsi transcripts of one- and ten-month-old goats were analyzed by sequencing RNAs and miRNAs. The results showed that the ten-month-old Longlin goats had 327 up- and 419 down-regulated differentially expressed genes (DEGs) compared with the one-month-old. In addition, 20 co-up-regulated and 55 co-down-regulated miRNAs involved in the muscle fiber hypertrophy of goats were identified in ten-month-old Longlin and Nubian goats compared with one-month-old. Five miRNA–mRNA pairs (chi-let-7b-3p-MIRLET7A, chi-miR193b-3p-MMP14, chi-miR-355-5p-DGAT2, novel_128-LOC102178119, novel_140-SOD3) involved in the goat skeletal muscle development were identified by miRNA–mRNA negative correlation network analysis. Our results provided new insight into the functional roles of goat muscle-associated miRNAs, allowing a deeper understanding of the transformation of miRNA roles during mammalian muscle development.

Published in Genes

ISSN: 2073-4425 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://www.mdpi.com/journal/genes/

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