Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification

Xiaoyu Xin; Xiaoyu Xin; Xiaoyu Xin; Chang Liu; Chang Liu; Chang Liu; Zhaoqun Liu; Zhaoqun Liu; Zhaoqun Liu; Yukun Zhang; Yukun Zhang; Yukun Zhang; Yuqian Gao; Yuqian Gao; Yuqian Gao; Ting Zhu; Ting Zhu; Ting Zhu; Lingling Wang; Lingling Wang; Lingling Wang; Lingling Wang; Linsheng Song; Linsheng Song; Linsheng Song; Linsheng Song

doi:10.3389/fmars.2022.1050022

Frontiers in Marine Science (Nov 2022)

Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification

Xiaoyu Xin,
Xiaoyu Xin,
Xiaoyu Xin,
Chang Liu,
Chang Liu,
Chang Liu,
Zhaoqun Liu,
Zhaoqun Liu,
Zhaoqun Liu,
Yukun Zhang,
Yukun Zhang,
Yukun Zhang,
Yuqian Gao,
Yuqian Gao,
Yuqian Gao,
Ting Zhu,
Ting Zhu,
Ting Zhu,
Lingling Wang,
Lingling Wang,
Lingling Wang,
Lingling Wang,
Linsheng Song,
Linsheng Song,
Linsheng Song,
Linsheng Song

Affiliations

Xiaoyu Xin: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Xiaoyu Xin: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Xiaoyu Xin: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Chang Liu: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Chang Liu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Chang Liu: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Zhaoqun Liu: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Zhaoqun Liu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Zhaoqun Liu: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Yukun Zhang: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Yukun Zhang: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Yukun Zhang: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Yuqian Gao: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Yuqian Gao: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Yuqian Gao: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Ting Zhu: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Ting Zhu: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Ting Zhu: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Lingling Wang: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Lingling Wang: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Lingling Wang: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Lingling Wang: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China
Linsheng Song: Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian, China
Linsheng Song: Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, China
Linsheng Song: Dalian Key Laboratory of Aquatic Animal Disease Prevention and Control, Dalian Ocean University, Dalian, China
Linsheng Song: Functional Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

DOI: https://doi.org/10.3389/fmars.2022.1050022
Journal volume & issue: Vol. 9

Abstract

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The biosynthesis of shell is a complicated calcification process in the marine bivalve, which can be severely impacted by ocean acidification (OA). Calmodulin (CaM) is a pivotal calcium regulator and thought to be crucial for calcification. In the present study, a CaM (designated CgCaM) with calcium-binding activity was identified from the Pacific oyster Crassostrea gigas with the objective to understand its possible role in the regulation of calcium homeostasis under acidification treatment. The open reading frame (ORF) of CgCaM was of 474 bp encoding a 17.5 kDa protein with four continuous EF-hand domains. CgCaM shared high similarity with CaMs from other invertebrates and vertebrates. The mRNA transcript of CgCaM was constitutively expressed in all detected tissues with the higher expression level in mantle, especially highest in the middle fold of the three folds of mantle. CgCaM was found to be mainly distributed in the mantle epithelium. When the oysters were exposed to acidified seawater, the expression level of CgCaM in the middle fold of mantle and the content of Ca2+ in this fold both decreased significantly. These results collectively suggested that CgCaM was involved in the regulation of calcium homeostasis in the middle fold of mantle under acidification treatment.

Published in Frontiers in Marine Science

ISSN: 2296-7745 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Natural history (General): General. Including nature conservation, geographical distribution
Website: https://www.frontiersin.org/journals/marine-science

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