Flavonoids Extraction Kinetics, Antimicrobial Activity and Radical Scavenging Potential of Bulgarian Woundwort (<i>Solidago virgaurea</i> L.)
Zvezdelina Yaneva,
Evgeni Simeonov,
Nikolina Rusenova,
Donika Ivanova,
Galina Nikolova,
Yanka Karamalakova,
Chavdar Chilev,
Georgi Beev
Affiliations
Zvezdelina Yaneva
Chemistry Unit, Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria
Evgeni Simeonov
Department of Chemical Engineering, Faculty of Chemical and System Engineering, University of Chemical Technology and Metallurgy, 1756 Sofia, Bulgaria
Nikolina Rusenova
Veterinary Microbiology Unit, Department of Veterinary Microbiology, Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria
Donika Ivanova
Chemistry Unit, Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria
Galina Nikolova
Department of Chemistry and Biochemistry, Faculty of Medicine, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria
Yanka Karamalakova
Department of Chemistry and Biochemistry, Faculty of Medicine, Trakia University, 11 Armeiska Str., 6000 Stara Zagora, Bulgaria
Chavdar Chilev
Department of Chemical Engineering, Faculty of Chemical and System Engineering, University of Chemical Technology and Metallurgy, 1756 Sofia, Bulgaria
Georgi Beev
Department of Biochemistry, Microbiology and Physics, Faculty of Agriculture, Trakia University, Students Campus, 6000 Stara Zagora, Bulgaria
The medicinal plant woundwort (Solidago virgaurea L.) characterizes by diuretic, antimutagenic, anti-inflammatory activity and it has been applied for urinary tract, nephrolithiasis and prostate disorders treatment. The aim of the present study was to analyze the extraction kinetics of catechin, epigallocatechin and quercetin from Bulgarian woundwort extracts, to assess the antibacterial potential of the medicinal plant extracts against four bacterial strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Bacillus cereus), their antioxidant capacity and radical scavenging potential. The concentrations of the flavonoids in the extracts obtained at different extraction conditions (solvent, temperature, extraction time) were determined by newly-developed by the scientific team RP-HPLC-PDA methodologies. The agar well diffusion method was applied to evaluate the antibacterial activity of the plant extracts. The 70% EtOH extracts at 20 °C displayed significantly higher antibacterial activity against the foodborne pathogenic bacteria S. aureus and P. aeruginosa as compared to the 70% and 98% EtOH extracts at 30 °C and 20 °C, respectively. The medicinal plant exhibited satisfactory antioxidant potential and radical-scavenging activity.
