IUCrJ (Sep 2019)
1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector
- A. Tolstikova,
- M. Levantino,
- O. Yefanov,
- V. Hennicke,
- P. Fischer,
- J. Meyer,
- A. Mozzanica,
- S. Redford,
- E. Crosas,
- N. L. Opara,
- M. Barthelmess,
- J. Lieske,
- D. Oberthuer,
- E. Wator,
- I. Mohacsi,
- M. Wulff,
- B. Schmitt,
- H. N. Chapman,
- A. Meents
Affiliations
- A. Tolstikova
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- M. Levantino
- European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France
- O. Yefanov
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- V. Hennicke
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- P. Fischer
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- J. Meyer
- Deutsches Elektronen Synchrotron, Photon Science, Notkestrasse 85, 22607 Hamburg, Germany
- A. Mozzanica
- Paul Scherrer Institute, 111 Forschungsstrasse, 5232 Villigen, Switzerland
- S. Redford
- Paul Scherrer Institute, 111 Forschungsstrasse, 5232 Villigen, Switzerland
- E. Crosas
- Deutsches Elektronen Synchrotron, Photon Science, Notkestrasse 85, 22607 Hamburg, Germany
- N. L. Opara
- Paul Scherrer Institute, 111 Forschungsstrasse, 5232 Villigen, Switzerland
- M. Barthelmess
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- J. Lieske
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- D. Oberthuer
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- E. Wator
- Malopolska Centre of Biotechnology, Jagiellonian University, Cracow 30-387, Poland
- I. Mohacsi
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- M. Wulff
- European Synchrotron Radiation Facility, 71 Avenue des Martyrs, 38000 Grenoble, France
- B. Schmitt
- Paul Scherrer Institute, 111 Forschungsstrasse, 5232 Villigen, Switzerland
- H. N. Chapman
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- A. Meents
- Center for Free Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany
- DOI
- https://doi.org/10.1107/S205225251900914X
- Journal volume & issue
-
Vol. 6,
no. 5
pp. 927 – 937
Abstract
Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.
Keywords
- serial crystallography
- synchrotron radiation
- pink beams
- protein crystallography
- protein structure
- structure determination
