Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.)

Zhansheng Wu; Zhansheng Wu; Yuanyuan Huang; Yan Li; Jiawei Dong; Xiaochen Liu; Chun Li

doi:10.3389/fmicb.2019.02676

Frontiers in Microbiology (Nov 2019)

Biocontrol of Rhizoctonia solani via Induction of the Defense Mechanism and Antimicrobial Compounds Produced by Bacillus subtilis SL-44 on Pepper (Capsicum annuum L.)

Zhansheng Wu,
Zhansheng Wu,
Yuanyuan Huang,
Yan Li,
Jiawei Dong,
Xiaochen Liu,
Chun Li

Affiliations

Zhansheng Wu: Department of Bioengineering, School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an, China
Zhansheng Wu: Department of Environmental and Biological Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China
Yuanyuan Huang: Department of Environmental and Biological Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China
Yan Li: Department of Environmental and Biological Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China
Jiawei Dong: Department of Environmental and Biological Engineering, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China
Xiaochen Liu: Department of Bioengineering, School of Environmental and Chemical Engineering, Xi’an Polytechnic University, Xi’an, China
Chun Li: Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing, China

DOI: https://doi.org/10.3389/fmicb.2019.02676
Journal volume & issue: Vol. 10

Abstract

Read online

Pepper seedling wilt disease is the main cause of crop yield reduction. Biocontrol agents are widely used to control plant diseases caused by pathogenic fungi and activate plant defense systems. Our preliminary work showed that Bacillus subtilis SL-44 played a significant role in the reduction of wilt disease severity on pepper plants. To evaluate biological control mechanism of B. subtilis SL-44 on wilt disease caused by Rhizoctonia solani, the activities of the related enzymes were detected in the pepper seedling with different treatment in this study. Fluorescence microscopy combined with different dyes showed that B. subtilis SL-44 induced a large amount of active oxygen and callose accumulation in pepper leaves. The defense-related enzyme activities in pepper were improved significantly when treated with B. subtilis SL-44, including peroxidase, catalase, superoxide dismutase, polyphenol oxidase, and phenylalanine ammonia lyase. The activity of chitinase and β-1,3-glucanase in B. subtilis SL-44-treated pepper was also enhanced. Furthermore, the expression level of pepper-resistance gene CaPIN II was significantly increased in B. subtilis SL-44 treatment. Besides, B. subtilis SL-44 filtrate led to the death of the pathogenic fungus by fracturing the mycelia and leaking of the cell contents. Surfactin, iturin, and fengycin were found in B. subtilis SL-44 crude extracts, which could be effective antifungal compounds against R. solani. The results suggest that B. subtilis SL-44 could not only activate induced systemic resistance of pepper seedling against wilt disease caused by R. solani by jasmonic acid-dependent signaling pathway but also produce antifungal compounds to inhibit or even damage the mycelium growth of R. solani. The findings of this study provide novel guidance in plant protection development.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

About the journal

Abstract

Keywords