Comparative Milk Clotting Potentials of Fusarium oxysporum and Aspergillus Niger Fungal Strains Isolated from Fermented Brown Rice

Abstract

The objective of this paper is to investigate the comparative milk clotting potentials of Fusarium oxysporum and Aspergillus niger fungal strains isolated from fermented using casein hydrolysis test initially and subsequently subjecting the fungal strains to fermentation on five agro-industrial waste and casein for detection of MCTs and MCAs. After fermentation, enzymes extracted from F.oxysporum treated with CS revealed highest MA (288.8SU/ml) at 416secs, followed by enzymes extracted from RB which had (160.8 SU/ml) at 750secs; but for A.niger enzymes extracted from CS and RB had lower MCA units as compared to F.oxysporum, however, enzymes of A.niger extracted from SBH yielded 205SU/ml at 583secs as against (51.67) at 2427secs for F.oxysporum enzymes from SBH. The pellet enzymes for both of the fungal strains had low MCA and high MCTs; indicating that clotting activity was more potential with supernatant enzymes across the substrates. Although 163.3 CS and 137.0 SBH pellet enzymes from A. niger had a bit high MCA and low MCTs as compared to pellet enzymes of F. oxysporum from 51.67 CS and 49.46 SBH. Production of enzymes using agro-industrial waste is a bioprocess that is cheaper and could add value economically.

Keywords

• Agro-industrial waste, Milk Clotting Potential; Fusarium oxysporum; Aspergillus niger; Fermented Brown Rice