Evaluation of Culture Results in Pediatric Clinics of the Training and Research Hospital

Abstract

Aim:Infections due to drug resistant microorganisms are increasing and it is important to evaluate culture specimens for early recognition, fast and effective therapy, epidemiological and prognosis of the infections. The aim of the study is to evaluate the results of blood and urine cultures taken from patients hospitalized in pediatric services outside the neonatal intensive care unit.Materials and Methods:In this study, 2277 blood cultures and 857 urine cultures taken between the years of 2007 and 2008 were evaluated retrospectively. 6.8% (n=156) positive blood cultures and 6% (n=52) positive urine cultures were included in the study. Blood cultures were put onto Bact-Alert 3D automotized blood culture systems and urine cultures were put onto MacConkey and 5% sheep blood agar plates. Microorganisms were identified with routine bacteriological procedures and antibiotic sensitivity tests were performed.Results:In pediatrics clinics, 79.4% gram positive microorganisms (69% coagulase-negative staphylococcus CNS) among positive blood cultures 86.5% gram negative microorganisms (61.5% Escherichia coli) among positive urine cultures were produced. Microorganisms grew in blood cultures were as follows: 48% (n=75) Methicillin-resistant CNS, 21% (n=33) Methicillin-sensitive CNS, 7.5% (n=12) Klebsiella species (4.5% ESBL positive), 2.6% (n=4) Streptococcus pneumoniae, 2.6% (n=4) Methicillin-sensitive staphylococci 2% (n=3) Acinetobacter, 2.6% (n=4) Candida tropicalis, 2% (n=3) Escherichia coli, 2% (n=3) Enterobacter, 1.3% (n=2) Haemophilus influenzae, 0.6% (n=1) Brucella spp. Microorganisms detected in urine cultures are as follows: 61.5% (n=32) Escherichia coli (40% ESBL positive), 11.5% (n=6) Enterococcus spp., 5.8% (n=3) Proteus spp., 3.8% (n=2) Pseudomonas spp., 3.8% (n=2) Stenotrophomonas maltophilia, 2% (n=1) Methicillin-sensitive staphylococci, 2% (n=1) Acinetobacter baumannii, 2% (n=1) Klebsiella spp.Conclusion:Identifying the infectious agents and their antibiotic susceptibility and resistance rates is important for adequate and effective initial empiric antimicrobial therapy and treatment of infections.

Keywords

• children
• bacteremia
• urinary tract infection