Nature Communications (Dec 2018)

3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening

  • Lorna J. Hale,
  • Sara E. Howden,
  • Belinda Phipson,
  • Andrew Lonsdale,
  • Pei X. Er,
  • Irene Ghobrial,
  • Salman Hosawi,
  • Sean Wilson,
  • Kynan T. Lawlor,
  • Shahnaz Khan,
  • Alicia Oshlack,
  • Catherine Quinlan,
  • Rachel Lennon,
  • Melissa H. Little

DOI
https://doi.org/10.1038/s41467-018-07594-z
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 17

Abstract

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Studies examining human podocytopathies have utilised 2D cultured primary or immortalised podocyte cell lines. Here, the authors demonstrate that 3D human glomeruli sieved from induced pluripotent stem cell-derived kidney organoids retain an improved podocyte identity in vitro facilitating disease modelling and toxicity testing.