Circulating and Endometrial Profiles of miR-145, miR-155-5p, miR-224, MPP-5, and PECAM-1 Expression in Patients with Repeated Implantation Failure: A Case Control Study
Jamileh Jahanbakhsh,
Maryam Farid Mojtahedi,
Nariman Moradi,
Reza Fadaei,
Afsaneh Tehranian,
Rahim Rostami,
Ladan Kashani,
Ashraf Moeini,
Shahin Alizadeh-Fanalou,
Sepideh Barzin Tond,
Mahshad Khodarahmian,
Soudabeh Fallah
Affiliations
Jamileh Jahanbakhsh
Infertility Ward, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Maryam Farid Mojtahedi
Infertility Ward, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Nariman Moradi
Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
Reza Fadaei
Sleep Disorders Research Centre, Kermanshah University of Medical Sciences, Kermanshah, Iran
Afsaneh Tehranian
Department of Gynaecology and Obstetrics, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Rahim Rostami
Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Ladan Kashani
Infertility Ward, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Ashraf Moeini
Infertility Ward, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Shahin Alizadeh-Fanalou
Department of Clinical Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia Iran
Sepideh Barzin Tond
Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Mahshad Khodarahmian
Embryology Laboratory, IVF Department, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Soudabeh Fallah
Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Objective: An association between microRNAs (miRNAs) and adhesion proteins expression with repeated implantationfailure (RIF) has been recently reported; however, these findings are controversial. This study aims to evaluatethe endometrial and circulating expressions of miR-145, miR-155-5p, and miR-224 in addition to the endometrialexpressions of membrane protein palmitoylated-5 (MPP-5) and endothelial cell adhesion molecule-1 (PECAM-1) inpatients with RIF compared to control subjects.Materials and Methods: This case-control study was carried out between June 2021-July 2022. Subjects included 17patients with RIF and 17 control subjects, who had previous spontaneous term pregnancy with a live birth, who referredto the Medical Centre of Arash Hospital, Tehran, Iran. Endometrial tissue samples were obtained via hysteroscopyand Pipelle catheter in the RIF and control subjects, respectively. Plasma samples were collected after ovulationin all subjects. The expression levels of MPP5, PECAM-1, miR-224, miR-145, and miR-155-5p were evaluated byquantitative real-time polymerase chain reaction (qRT-PCR). The student’s t test, chi-square, Mann-Whitney U, andanalysis of covariance (ANCOVA) were used for data analyses.Results: RIF patients had less endometrial miR-155-5p expression, and higher endometrial and circulating expressions ofmiR-145 and miR-224 compared to control subjects. Endometrial PECAM-1 and MPP5 expression significantly decreased inpatients with RIF compared to the control group. There was a positive correlation between circulating miR-224 and endometrialmiR-155-5p, and between circulating miR-155-5p and endometrial PECAM-1 expression levels in patients with RIF.Conclusion: The present study suggests that circulating miR-224, endometrial miR-145, and PECAM-1 can bereliable, novel biomarkers for diagnosis of RIF.
