Phytochemical Identification, Acute, and Sub-Acute Oral Toxicity Studies of the Foliar Extract of <i>Withania frutescens</i>
Abdelfattah EL Moussaoui,
Mohammed Bourhia,
Fatima Zahra Jawhari,
Hamza Mechchate,
Meryem Slighoua,
Ahmed Bari,
Riaz Ullah,
Hafiz Majid Mahmood,
Syed Saeed Ali,
Samir Ibenmoussa,
Dalila Bousta,
Amina Bari
Affiliations
Abdelfattah EL Moussaoui
Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez BP.1796, Morocco
Mohammed Bourhia
Laboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy, University of Casablanca, Casablanca BP.5696, Morocco
Fatima Zahra Jawhari
Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez BP.1796, Morocco
Hamza Mechchate
Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez BP.1796, Morocco
Meryem Slighoua
Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez BP.1796, Morocco
Ahmed Bari
Department of Pharmaceutical Chemistry and Central Laboratory, College of Pharmacy King Saud University, Riyadh 11451, Saudi Arabia
Riaz Ullah
Department of Pharmacognosy (MAPPRC), College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Hafiz Majid Mahmood
Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Syed Saeed Ali
Department of Pharmaceutical Chemistry and Central Laboratory, College of Pharmacy King Saud University, Riyadh 11451, Saudi Arabia
Samir Ibenmoussa
Laboratory of Chemistry-Biochemistry, Environment, Nutrition, and Health, Faculty of Medicine and Pharmacy, University of Casablanca, Casablanca BP.5696, Morocco
Dalila Bousta
Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez BP.1796, Morocco
Amina Bari
Laboratory of Biotechnology, Environment, Agrifood and Health, Faculty of Science, University of Sidi Mohamed Ben Abdellah, Fez BP.1796, Morocco
Withania frutescens (W. frutescens) is a medicinal plant widely used to treat several diseases. This work aims to study phytochemical composition as well as acute and subacute toxicity of W. frutescens hydroethanolic extract in mice. The phytochemical composition of W. frutescens extract was performed using gas chromatographic analysis. Acute toxicity was studied in vivo with oral administration of single doses 400 mg/kg, 1000 mg/kg, and 2000 mg/kg for 14 days. Subacute toxicity was studied with the administration of repeated doses of 400 mg/kg/day and 2000 mg/kg/day for 28 days. Phytochemical analysis of W. frutescens hydro-ethanolic extract confirmed the presence of interesting chemical compounds. Acute toxicity results showed no toxic symptoms in mice treated with an increasing dose up to a maximum of 2000 mg/kg. Alongside acute toxicity, subacute data showed no clinical symptoms nor biochemical or histological alteration in mice treated with an increasing dose up to a maximum of 2000 mg/kg compared to the control group (p W. frutescens extract, and, therefore, this plant can be considered safe in animals up to 2000 mg/kg under both acute and subacute toxicity conditions.
