The Effect of L-Ascorbic Acid-2-Phosphate Magnesium on Chicks Injected a Radical Initiator

Abstract

Effect of L-ascorbic acid phosphate magnesium (APM) on chicks injected radical initiator, 2, 2′-azobis(2-amidinopropane) dihydrochloride (AAPH), was examined. Chicks were fed APM as ascorbate resource for 7 days. Birds were administrated AAPH by intraperitoneal injection after APM feeding. The changes of ascorbate and thiobarbituric acid reactive substances (TBARS) in plasma and liver were determined before, and 3 and 6 hours after AAPH injection. Both plasma and liver ascorbate increased by APM feeding. After AAPH injection, liver ascorbate decreased, but plasma ascorbate increased in both control and APM-fed birds. AAPH administration raised liver TBARS, but decreased plasma TBARS. Both plasma and liver TBARS were lower in APM group than control group before and after AAPH administration. Peroxyl radical trapping activity in plasma was elevated by APM feeding as compared to control group. APM was utilized as ascorbate, and was effective for prevention of oxidative stresses derived from AAPH by increasing plasma and liver ascorbate and plasma radical trapping activity.

Keywords

• chick
• l-ascorbic acid-2-phosphate magnesium
• radical initiator
• oxidative stress