LRRC45 Is a Centrosome Linker Component Required for Centrosome Cohesion
Runsheng He,
Ning Huang,
Yitian Bao,
Haining Zhou,
Junlin Teng,
Jianguo Chen
Affiliations
Runsheng He
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China
Ning Huang
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China
Yitian Bao
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China
Haining Zhou
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China
Junlin Teng
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China
Jianguo Chen
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State, Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, China
During interphase, centrosomes are connected by a proteinaceous linker between the proximal ends of the centrioles, which is important for the centrosomes to function as a single microtubule-organizing center. However, the composition and regulation of centrosomal linker remain largely unknown. Here, we show that LRRC45 is a centrosome linker that localizes at the proximal ends of the centrioles and forms fiber-like structures between them. Depletion of LRRC45 results in centrosome splitting during interphase. Moreover, LRRC45 interacts with both C-Nap1 and rootletin and is phosphorylated by Nek2A at S661 during mitosis. After phosphorylation, both LRRC45 centrosomal localization and fiber-like structures are significantly reduced, which subsequently leads to centrosome separation. Thus, LRRC45 is a critical component of the proteinaceous linker between two centrioles and is required for centrosome cohesion.
