Hyocholic acid as internal standard for quantitation of human fecal bile acids

Abstract

After hyocholate is added to a fecal homogenate, the bile acids are extracted, purified by thin-layer chromatography, and quantitated by gas–liquid chromatography as methyl ester trifluoroacetates on QF-1 columns.

Keywords

• gas–liquid chromatography
• trifluoroacetates