Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors

Yang Liu; Filipe Pinto; Xinyi Wan; Zhugen Yang; Shuguang Peng; Mengxi Li; Jonathan M. Cooper; Zhen Xie; Christopher E. French; Baojun Wang

doi:10.1038/s41467-022-29604-x

Nature Communications (Apr 2022)

Reprogrammed tracrRNAs enable repurposing of RNAs as crRNAs and sequence-specific RNA biosensors

Yang Liu,
Filipe Pinto,
Xinyi Wan,
Zhugen Yang,
Shuguang Peng,
Mengxi Li,
Jonathan M. Cooper,
Zhen Xie,
Christopher E. French,
Baojun Wang

Affiliations

Yang Liu: College of Chemical and Biological Engineering & Hangzhou Innovation Center, Zhejiang University
Filipe Pinto: Centre for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh
Xinyi Wan: Centre for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh
Zhugen Yang: Research Centre for Biological Computation, Zhejiang Laboratory
Shuguang Peng: Center for Synthetic and System Biology, Department of Automation, Beijing National Research Centre for Information Science and Technology, Tsinghua University
Mengxi Li: Centre for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh
Jonathan M. Cooper: Division of Biomedical Engineering, James Watt School of Engineering, University of Glasgow
Zhen Xie: Center for Synthetic and System Biology, Department of Automation, Beijing National Research Centre for Information Science and Technology, Tsinghua University
Christopher E. French: Centre for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh
Baojun Wang: College of Chemical and Biological Engineering & Hangzhou Innovation Center, Zhejiang University

DOI: https://doi.org/10.1038/s41467-022-29604-x
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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In type II CRISPR systems, the guide RNA (gRNA) comprises a CRISPR RNA (crRNA) and a hybridized trans-acting CRISPR RNA (tracrRNA), both being essential in guided DNA targeting functions. Here the authors investigate the programmability of crRNA-tracrRNA hybridization for Cas9 and apply this to biosensing.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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