Frontiers in Genetics (Jan 2023)
Mesoangioblasts at 20: From the embryonic aorta to the patient bed
- Giulio Cossu,
- Giulio Cossu,
- Giulio Cossu,
- Rossana Tonlorenzi,
- Silvia Brunelli,
- Maurilio Sampaolesi,
- Maurilio Sampaolesi,
- Graziella Messina,
- Emanuele Azzoni,
- Sara Benedetti,
- Stefano Biressi,
- Chiara Bonfanti,
- Laricia Bragg,
- Jordi Camps,
- Ornella Cappellari,
- Marco Cassano,
- Fabio Ciceri,
- Marcello Coletta,
- Diego Covarello,
- Stefania Crippa,
- M. Gabriella Cusella-De Angelis,
- Luciana De Angelis,
- Arianna Dellavalle,
- Jordi Diaz-Manera,
- Daniela Galli,
- Francesco Galli,
- Cesare Gargioli,
- Mattia F. M. Gerli,
- Giorgia Giacomazzi,
- Beatriz G. Galvez,
- Hidetoshi Hoshiya,
- Maria Guttinger,
- Anna Innocenzi,
- M. Giulia Minasi,
- Laura Perani,
- Stefano C Previtali,
- Mattia Quattrocelli,
- Martina Ragazzi,
- Urmas Roostalu,
- Giuliana Rossi,
- Raffaella Scardigli,
- Dario Sirabella,
- Francesco Saverio Tedesco,
- Yvan Torrente,
- Gonzalo Ugarte
Affiliations
- Giulio Cossu
- Division of Cell Matrix Biology and Regenerative Medicine. University of Manchester, Manchester, United Kingdom
- Giulio Cossu
- Division of Neuroscience, IRCCS Ospedale San Raffaele, Milan, Italy
- Giulio Cossu
- Muscle Research Unit, Charité Medical Faculty and Max Delbrück Center, Berlin, Germany
- Rossana Tonlorenzi
- Division of Neuroscience, IRCCS Ospedale San Raffaele, Milan, Italy
- Silvia Brunelli
- School of Medicine and Surgery, University of Milano Bicocca, Milan, Italy
- Maurilio Sampaolesi
- Translational Cardiomyology Laboratory, Stem Cell and Developmental Biology Unit, Department of Development and Regeneration, KU Leuven, Leuven, Belgium
- Maurilio Sampaolesi
- Histology and Medical Embryology Unit, Department of Anatomy, Forensic Medicine and Orthopaedics, Sapienza University, Rome, Italy
- Graziella Messina
- Department of Biosciences, University of Milan, Milan, Italy
- Emanuele Azzoni
- School of Medicine and Surgery, University of Milano Bicocca, Milan, Italy
- Sara Benedetti
- UCL Great Ormond Street Institute of Child Health and NIHR GOSH Biomedical Research Centre, London, United Kingdom
- Stefano Biressi
- Department of Cellular, Computational and Integrative Biology (CIBIO) and Dulbecco Telethon Institute, University of Trento, Trento, Italy
- Chiara Bonfanti
- Department of Biosciences, University of Milan, Milan, Italy
- Laricia Bragg
- Division of Cell Matrix Biology and Regenerative Medicine. University of Manchester, Manchester, United Kingdom
- Jordi Camps
- 0Bayer AG, Research and Development, Pharmaceuticals, Berlin, Germany
- Ornella Cappellari
- 1Department of Pharmacy-Drug Sciences, University of Bari “Aldo Moro”, Bari, Italy
- Marco Cassano
- 2Lunaphore Technologies SA, Tolochenaz, Switzerland
- Fabio Ciceri
- Division of Neuroscience, IRCCS Ospedale San Raffaele, Milan, Italy
- Marcello Coletta
- Histology and Medical Embryology Unit, Department of Anatomy, Forensic Medicine and Orthopaedics, Sapienza University, Rome, Italy
- Diego Covarello
- 3AGC Biologicals, Milan, Italy
- Stefania Crippa
- 4San Raffaele-Telethon Institute of Gene Theray, IRCCS Ospedale San Raffaele, Milan, Italy
- M. Gabriella Cusella-De Angelis
- 5Department of Anatomy, University of Pavia, Pavia, Italy
- Luciana De Angelis
- Histology and Medical Embryology Unit, Department of Anatomy, Forensic Medicine and Orthopaedics, Sapienza University, Rome, Italy
- Arianna Dellavalle
- 6Cord Blood Bank, InScientiaFides, San Marino
- Jordi Diaz-Manera
- 7John Walton Muscular Dystrophy Research Centre, Newcastle University, United Kingdom
- Daniela Galli
- 8Department of Medicine and Surgery, University of Parma, Parma, Italy
- Francesco Galli
- Division of Cell Matrix Biology and Regenerative Medicine. University of Manchester, Manchester, United Kingdom
- Cesare Gargioli
- 9Department of Biology, University of Tor Vergata, Rome, Italy
- Mattia F. M. Gerli
- 0UCL Department of Surgical Biotechnology and Great Ormond Street Institute of Child Health, London, United Kingdom
- Giorgia Giacomazzi
- 1CellCarta, Gosselies, Belgium
- Beatriz G. Galvez
- 2Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Universidad Complutense de Madrid, Madrid, Spain
- Hidetoshi Hoshiya
- 3CellFiber Co., Ltd, Tokyo, Japan
- Maria Guttinger
- 4IFO, Istituti Fisioterapici Ospedalieri, Rome, Italy
- Anna Innocenzi
- Division of Neuroscience, IRCCS Ospedale San Raffaele, Milan, Italy
- M. Giulia Minasi
- 5Lavitaminasi, Clinical Nutrition and Reproductive Medicine, Rome, Italy
- Laura Perani
- Division of Neuroscience, IRCCS Ospedale San Raffaele, Milan, Italy
- Stefano C Previtali
- Division of Neuroscience, IRCCS Ospedale San Raffaele, Milan, Italy
- Mattia Quattrocelli
- 6Division of Molecular Cardiovascular Biology, University of Cincinnati, Cincinnati, OH, United States
- Martina Ragazzi
- 7Gubra ApS, Horsholm, Denmark
- Urmas Roostalu
- 8Roche Institute for Translational Bioengineering (ITB), pRED Basel, Basel, Switzerland
- Giuliana Rossi
- 9Institute of Translational Pharmacology, National Research Council, Rome, Italy
- Raffaella Scardigli
- 0Columbia Stem Cell Initiative, Department of Rehabilitation and Regenerative Medicine, Columbia University, New York, United States
- Dario Sirabella
- 1University College London, Great Ormond Street Hospital for Children and the Francis Crick Institute, London, United Kingdom
- Francesco Saverio Tedesco
- 2Laboratory of Neuroscience, Faculty of Chemistry and Biology, University of Santiago de Chile, Santiago, Chile
- Yvan Torrente
- UCL Great Ormond Street Institute of Child Health and NIHR GOSH Biomedical Research Centre, London, United Kingdom
- Gonzalo Ugarte
- 2Laboratory of Neuroscience, Faculty of Chemistry and Biology, University of Santiago de Chile, Santiago, Chile
- DOI
- https://doi.org/10.3389/fgene.2022.1056114
- Journal volume & issue
-
Vol. 13
Abstract
In 2002 we published an article describing a population of vessel-associated progenitors that we termed mesoangioblasts (MABs). During the past decade evidence had accumulated that during muscle development and regeneration things may be more complex than a simple sequence of binary choices (e.g., dorsal vs. ventral somite). LacZ expressing fibroblasts could fuse with unlabelled myoblasts but not among themselves or with other cell types. Bone marrow derived, circulating progenitors were able to participate in muscle regeneration, though in very small percentage. Searching for the embryonic origin of these progenitors, we identified them as originating at least in part from the embryonic aorta and, at later stages, from the microvasculature of skeletal muscle. While continuing to investigate origin and fate of MABs, the fact that they could be expanded in vitro (also from human muscle) and cross the vessel wall, suggested a protocol for the cell therapy of muscular dystrophies. We tested this protocol in mice and dogs before proceeding to the first clinical trial on Duchenne Muscular Dystrophy patients that showed safety but minimal efficacy. In the last years, we have worked to overcome the problem of low engraftment and tried to understand their role as auxiliary myogenic progenitors during development and regeneration.
Keywords
