Mitigating the non-specific uptake of immunomagnetic microparticles enables the extraction of endothelium from human fat

Abstract

Antonyshyn et al. establish a methodology for acquiring human adipose tissue-derived microvascular endothelial cells that can be readily applied in future vascular tissue engineering applications. The authors developed strategies to mitigate the non-specific uptake of immunomagnetic microparticles to facilitate the immunoselection of endothelial cells by magnet-assisted cell sorting.