PLoS ONE (Jan 2013)

Ubiquitin-specific peptidase 5, a target molecule of vialinin A, is a key molecule of TNF-α production in RBL-2H3 cells.

  • Yasukiyo Yoshioka,
  • Yue Qi Ye,
  • Kiyoshi Okada,
  • Kayoko Taniguchi,
  • Ayaka Yoshida,
  • Kouichi Sugaya,
  • Jun-ichi Onose,
  • Hiroyuki Koshino,
  • Shunya Takahashi,
  • Arata Yajima,
  • Shunsuke Yajima,
  • Naoki Abe

DOI
https://doi.org/10.1371/journal.pone.0080931
Journal volume & issue
Vol. 8, no. 12
p. e80931

Abstract

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Tumor necrosis factor alpha (TNF-α), a central mediator of the inflammatory response, is released from basophilic cells and other cells in response to a variety of proinflammatory stimuli. Vialinin A is a potent inhibitor of TNF-α production and is released from RBL-2H3 cells. Ubiquitin-specific peptidase 5 (USP5), a deubiquitinating enzyme, was identified as a target molecule of vialinin A and its enzymatic activity was inhibited by vialinin A. Here we report production of TNF-α is decreased in USP5 siRNA-knockdown RBL-2H3 cells, compared with control cells. The finding of the present study strongly suggests that USP5 is one of the essential molecules for the production of TNF-α in RBL-2H3.