Chemical and Biological Technologies in Agriculture (Apr 2018)

Genetic diversity, microbiological study and composition of soil associated with wild Pleurotus ostreatus from different locations in Ondo and Ekiti States, Nigeria

  • Taye Victor Familoni,
  • Clement Olusola Ogidi,
  • Bamidele Juliet Akinyele,
  • Anthony Kayode Onifade

DOI
https://doi.org/10.1186/s40538-018-0119-y
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 12

Abstract

Read online

Abstract Background Genetic variation among different strains of wild macrofungi can be expressed and documented using different molecular tools. In this study, the genetic diversity and relatedness of Pleurotus ostreatus from different locations in Ondo and Ekiti States were investigated. Methods Random amplified polymorphic DNA-polymerase chain reaction marker was adopted to assess the genetic diversity of some wild P. ostreatus. Microorganisms associated with the wild P. ostreatus and their soil (substrate) were enumerated and identified using standard microbiological methods, while composition of soil around the mushrooms was evaluated using the conventional method. Results A total of 114 positive DNA bands were observed among wild P. ostreatus based on random amplified polymorphic DNA analysis (RAPD) with 10 primers, showing a total number of polymorphic markers of 91 and average polymorphism of 80.24%. P. ostreatus from Ala quarters and Igbatoro Road in Akure related to P. ostreatus from Ado-Ekiti, while P. ostreatus from Ido-Ekiti and Usi-Ekiti are genetically similar. The bacterial count from wild P. ostreatus and associated soil ranged from 1.20 × 104 to 4.70 × 105 and 1.70 × 107 to 6.10 × 108 CFU g−1, respectively. The highest fungal count of 3.40 × 104 and 7.40 × 107 SFU g−1 were obtained for wild P. ostreatus and associated soil at Usi-Ekiti, which possesses the highest organic content (5.90%). Isolated microorganisms were Pseudomonas putida, Streptomyces spp., Trichoderma spp., Penicillium italicum and others. The highest crude fiber (25.04%) and protein content (24.07%) were obtained in wild P. ostreatus from Usi-Ekiti. Conclusion RAPD revealed the genetic relatedness and genetic diversity among studied wild P. ostreatus, indigenous to two sates in Southwestern Nigeria. This will improve the strain selection for further utilization and documentation.

Keywords