Jichu yixue yu linchuang (Apr 2023)

Decitabine combined with arsenic trioxide inhibits proliferation and promotes apoptosis of acute myeloid leukemia cell lines

  • LIU Yue, CAO Yang, GU Weiying, SHANG Limei, LIU Yan

DOI
https://doi.org/10.16352/j.issn.1001-6325.2023.04.0608
Journal volume & issue
Vol. 43, no. 4
pp. 608 – 614

Abstract

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Objective To investigate the effects of decitabine (DAC) combined with arsenic trioxide (ATO) on proliferation and apoptosis of AML cells and its underlying molecular mechanism. Methods AML cell lines were treated with DAC and ATO alone or in combination. CCK8 was used to detect cell viability;Compusyn software was used to analyze the synergistic effects of the combination treatment;Flow cytometry was applied to determine the apoptosis and cell cycle distribution;Western blot was applied to detect the expressions of poly (ADP-ribose) polymerase (PARP), c-PARP, phosphatidylinositol 3-kinase (PI3K), p-PI3K, protein kinase B (Akt) and p-Akt. Results DAC and ATO alone inhibited AML cell proliferation in a concentration-dependent manner. The combination of the two drugs significantly inhibited cell proliferation, induced apoptosis and cycle arrest of AML cells (P＜0.05). Combination therapy reduced the phosphorylation level of PI3K and Akt (P＜0.05). Conclusions DAC combined with ATO may have anti-AML effect by inhibiting PI3K/Akt pathway, which provides new theoretical basis of DAC+ATO strategy for AML.

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