Understanding m6A changes in chromophobe renal cell carcinoma and predicting patient outcomes survival
Zhigang Chen,
Junbo Yang,
Wei Zhang,
Yang Qian,
Nan Zhang,
Zixin Chen,
Min Lu,
Liyuan Ge,
Cheng Liu,
Xiaojun Tian,
Guifang Jia,
Lulin Ma,
Baoguo Li
Affiliations
Zhigang Chen
Department of Urology, Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital)
Junbo Yang
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Wei Zhang
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Yang Qian
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Nan Zhang
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Zixin Chen
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Min Lu
Department of Urology, Peking University Third Hospital
Liyuan Ge
Department of Urology, Peking University Third Hospital
Cheng Liu
Department of Urology, Peking University Third Hospital
Xiaojun Tian
Department of Urology, Peking University Third Hospital
Guifang Jia
Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University
Lulin Ma
Department of Urology, Peking University Third Hospital
Baoguo Li
Department of Urology, Beijing Haidian Hospital (Haidian Section of Peking University Third Hospital)
Abstract N6-methyladenosine (m6A) is a prevalent mRNA modification known for its implications in various cancer types, yet its role in chromophobe renal cell carcinoma (chRCC) remains largely unexplored. In this study, we performed m6A-SEAL-seq and RNA-seq analyses on tissues from three chRCC subjects, aiming to uncover m6A alterations in chRCC. Our findings revealed reduced expression levels of four m6A regulators in chRCC tissues and highlighted differences in m6A levels compared to normal tissues. Furthermore, we identified specific genes and cancer-related pathways affected by these differences, including notable candidates like NOTCH1 and FGFR1, implicated in chRCC development. Additionally, we developed a predictive model based on the expression level of m6A associated genes, demonstrating promising prognostic capabilities for patient survival prediction. Overall, our study provides valuable insights into the role of m6A in chRCC and its potential as a prognostic indicator.
