Nature Communications (May 2020)

The mutational impact of culturing human pluripotent and adult stem cells

  • Ewart Kuijk,
  • Myrthe Jager,
  • Bastiaan van der Roest,
  • Mauro D. Locati,
  • Arne Van Hoeck,
  • Jerome Korzelius,
  • Roel Janssen,
  • Nicolle Besselink,
  • Sander Boymans,
  • Ruben van Boxtel,
  • Edwin Cuppen

DOI
https://doi.org/10.1038/s41467-020-16323-4
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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Genetic changes acquired during in vitro culture pose a challenge to application of stem cells. Here the authors use whole genome sequencing to show that cultured human adult and pluripotent stem cells have a high mutational load caused by oxidative stress and reduced oxygen tension in culture lowers mutation rates.