Morphological and molecular identification of Culicidae mosquitoes (Diptera: Culicidae) in Lorestan province, Western Iran
Mohammad Hassan Kayedi,
Fariba Sepahvand,
Ehsan Mostafavi,
Sadegh Chinikar,
Hamid Mokhayeri,
Ali Chegheni Sharafi,
Gary Wong,
Nariman Shahhosseini,
Seyed Hassan Moosa Kazemi
Affiliations
Mohammad Hassan Kayedi
Razi Herbal Medicines Research Center and Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Fariba Sepahvand
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Ehsan Mostafavi
Department of Epidemiology and Biostatistics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran
Sadegh Chinikar
Pasteur Institute of Iran, Tehran, Iran
Hamid Mokhayeri
Department of Communicable Diseases Control and Prevention, Health Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Ali Chegheni Sharafi
Department of Communicable Diseases Control and Prevention, Health Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Gary Wong
Département de Microbiologie-Infectiologie et d'Immunologie, Université Laval, Québec City, Québec, Canada; Pasteur Institute of Shanghai, China
Nariman Shahhosseini
Département de Microbiologie-Infectiologie et d'Immunologie, Université Laval, Québec City, Québec, Canada; Bernhard Nocht Institute for Tropical Medicine, WHO Collaborating Centre for Arbovirus and Hemorrhagic Fever Reference and Research, Hamburg, Germany; Corresponding author.
Seyed Hassan Moosa Kazemi
Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Corresponding author.
Culicidae mosquitoes are main vectors of arboviruses that cause arboviral diseases in humans. Studies on fauna, ecology, biology, resting behaviors of Culicidae mosquitoes are important and greatly impacts the control of arboviral diseases that are transmitted by vectors. The aim of the present study was to determine fauna of mosquitoes (Diptera: Culicidae) based on morphological and molecular (genomic) identification and their habitats in Lorestan province, Western Iran. Meanwhile mosquito samples were examined for arbovirus infection.Culicidae mosquitoes were caught in 2015 and 2016 from human homes, animal dwellings, storehouses and pit shelters in Lorestan province, Western Iran, using an oral aspirator (hand catch), total catch, human and animal bait and light trap methods. The samples were identified on the genus and species. Six species of Culex and eight species of Anopheles were caught. One complex species (Cx. pipiens complex) and a hybrid between Cx. pipiens pipiens biotype pipiens and Cx. pipiens pipiens biotype molestus were identified. Among all of the trapped mosquitoes (4211), 94.68% were from genus Culex mosquitoes (3987), which indicate that this genus is the dominant in Lorestan province, Western Iran. Anopheles comprised of 201 individuals out of the total catch. Arboviruses were not detected in these samples.
