The Contribution of S100B Suppression by Arundic Acid to the Inhibition of the Glio-Toxicity Induced by Beta-Amyloid in an Astrocytes Culture

Abstract

Background & Objective: It has been shown that glial activation has important role in the pathophysiology of Alzheimer’s disease. S100B is an astrocyte specific factor with deleterious effects on the neuronal and non-neuronal cells in the central nervous system. Arundic acid is an agent that inhibits the secretion and production of S100B in astrocytes. Therefore, we aimed to evaluate the contribution of S100B in the cyto-protective effects of Arundic acid against beta-amyloid in 1321N1 astrocyte cell culture. Materials & Methods: Human astrocyte cells (1321N1) were treated with beta-amyloid (200 μM) and / or Arundic acid (50 μM) for 24 hours. Cell viability was measured using the MTT (3, 4, 5-dimethylthiazole-2, 5-diphenyl tetrazolium bromide) method. The S100B protein level was measured by the ELISA method. Results: Beta-amyloid treatment reduced cell survival compared to the control-treated groups. In contrast, the addition of Arundic acid to beta-amyloid suppressed the beta-amyloid-induced cell death. Beta-amyloid also increased the S100B protein level. However, Arundic acid prevented the rise of S100B protein level induced by beta-amyloid. Conclusion: The reduction of S100B protein secretion may be involved in the protective effects of Arundic acid against the beta-amyloid induced Glio-toxicity in the astrocyte culture.

Keywords

• alzheimer disease
• amyloid beta-peptides
• s-100 calcium binding protein beta subunit
• arundic acid
• astrocytes