Chlorine inhalation induces acute chest syndrome in humanized sickle cell mouse model and ameliorated by postexposure hemopexin
Ammar Saadoon Alishlash,
Muna Sapkota,
Israr Ahmad,
Kelsey Maclin,
Noor A. Ahmed,
Adam Molyvdas,
Stephen Doran,
Carolyn J. Albert,
Saurabh Aggarwal,
David A. Ford,
Namasivayam Ambalavanan,
Tamas Jilling,
Sadis Matalon
Affiliations
Ammar Saadoon Alishlash
Department of Pediatrics, School of Medicine, University of Alabama at Birmingham, AL, USA; Corresponding author. Department of Pediatrics, University of Alabama at Birmingham, 1600 7th Ave S, Lowder 620, Birmingham, AL, 35233, USA.
Muna Sapkota
Department of Pediatrics, School of Medicine, University of Alabama at Birmingham, AL, USA
Israr Ahmad
Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, AL, USA
Kelsey Maclin
Department of Environmental Health Sciences, School of Public Health, University of Alabama at Birmingham, AL, USA
Noor A. Ahmed
Department of Clinical and Diagnostic Sciences, School of Health Professions, University of Alabama at Birmingham, AL, USA
Adam Molyvdas
Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, AL, USA
Stephen Doran
Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, AL, USA
Carolyn J. Albert
Saint Louis University Department of Biochemistry and Molecular Biology, USA
Saurabh Aggarwal
Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, AL, USA
David A. Ford
Saint Louis University Department of Biochemistry and Molecular Biology, USA
Namasivayam Ambalavanan
Department of Pediatrics, School of Medicine, University of Alabama at Birmingham, AL, USA
Tamas Jilling
Department of Pediatrics, School of Medicine, University of Alabama at Birmingham, AL, USA; Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, AL, USA
Sadis Matalon
Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Alabama at Birmingham, AL, USA
Triggering factors of Acute Chest Syndrome (ACS) is a leading cause of death in patients with Sickle Cell Disease (SCD) and targeted therapies are limited. Chlorine (Cl2) inhalation happens frequently, but its role as a potential trigger of ACS has not been determined. In this study, we hypothesized that Cl2 exposure resembling that in the vicinity of industrial accidents induces acute hemolysis with acute lung injury, reminiscent of ACS in humanized SCD mice. When exposed to Cl2 (500 ppm for 30 min), 64% of SCD mice succumbed within 6 h while none of the control mice expressing normal human hemoglobin died (p<0.01). Surviving SCD mice had evidence of acute hemolysis, respiratory acidosis, acute lung injury, and high concentrations of chlorinated palmitic and stearic acids (p<0.05) in their plasmas and RBCs compared to controls. Treatment with a single intraperitoneal dose of human hemopexin 30 min after Cl2 inhalation reduced mortality to around 15% (p<0.01) with reduced hemolysis (decreased RBCs fragility (p<0.001) and returned plasma heme to normal levels (p<0.0001)), improved oxygenation (p<0.0001) and reduced acute lung injury scores (p<0.0001). RBCs from SCD mice had significant levels of carbonylation (which predisposes RBCs to hemolysis) 6 h post-Cl2 exposure which were absent in RBCs of mice treated with hemopexin. To understand the mechanisms leading to carbonylation, we incubated RBCs from SCD mice with chlorinated lipids and identified sickling and increased hemolysis compared to RBCs obtained from control mice and treated similarly. Our study indicates that Cl2 inhalation induces ACS in SCD mice via induction of acute hemolysis, and that post exposure administration of hemopexin reduces mortality and lung injury. Our data suggest that SCD patients are vulnerable in Cl2 exposure incidents and that hemopexin is a potential therapeutic agent.
