Nature Communications (May 2020)
Unique properties of a subset of human pluripotent stem cells with high capacity for self-renewal
- Kevin X. Lau,
- Elizabeth A. Mason,
- Joshua Kie,
- David P. De Souza,
- Joachim Kloehn,
- Dedreia Tull,
- Malcolm J. McConville,
- Andrew Keniry,
- Tamara Beck,
- Marnie E. Blewitt,
- Matthew E. Ritchie,
- Shalin H. Naik,
- Daniela Zalcenstein,
- Othmar Korn,
- Shian Su,
- Irene Gallego Romero,
- Catrina Spruce,
- Christopher L. Baker,
- Tracy C. McGarr,
- Christine A. Wells,
- Martin F. Pera
Affiliations
- Kevin X. Lau
- Department of Anatomy and Neuroscience, University of Melbourne
- Elizabeth A. Mason
- Department of Anatomy and Neuroscience, University of Melbourne
- Joshua Kie
- Department of Anatomy and Neuroscience, University of Melbourne
- David P. De Souza
- Metabolomics Australia, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne
- Joachim Kloehn
- Department of Biochemistry and Molecular Biology, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne
- Dedreia Tull
- Metabolomics Australia, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne
- Malcolm J. McConville
- Metabolomics Australia, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne
- Andrew Keniry
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Tamara Beck
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Marnie E. Blewitt
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Matthew E. Ritchie
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Shalin H. Naik
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Daniela Zalcenstein
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Othmar Korn
- Australian Institute for Bioengineering and Nanotechnology, The University of Queensland
- Shian Su
- Division of Molecular Medicine, The Walter and Eliza Hall Institute
- Irene Gallego Romero
- Melbourne Integrative Genomics, School of Biosciences, University of Melbourne
- Catrina Spruce
- The Jackson Laboratory
- Christopher L. Baker
- The Jackson Laboratory
- Tracy C. McGarr
- The Jackson Laboratory
- Christine A. Wells
- Department of Anatomy and Neuroscience, University of Melbourne
- Martin F. Pera
- Department of Anatomy and Neuroscience, University of Melbourne
- DOI
- https://doi.org/10.1038/s41467-020-16214-8
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 18
Abstract
Human pluripotent cells (hPSCs) in standard culture are similar to mouse epiblast cells, but heterogeneity within hPSC cultures complicates comparisons. Here the authors show that a subpopulation of hPSCs enriched for self-renewal capacity have distinct cell cycle, metabolic, DNA methylation, and ATAC-seq profiles.
