Frontiers in Plant Science (Dec 2023)
Raffinose degradation-related gene GhAGAL3 was screened out responding to salinity stress through expression patterns of GhAGALs family genes
- Wenhua Chen,
- Wenhua Chen,
- Yupeng Cui,
- Yunxin He,
- Lanjie Zhao,
- Ruifeng Cui,
- Xiaoyu Liu,
- Hui Huang,
- Yuexin Zhang,
- Yapeng Fan,
- Xixian Feng,
- Kesong Ni,
- Tiantian Jiang,
- Mingge Han,
- Yuqian Lei,
- Mengyue Liu,
- Yuan Meng,
- Xiugui Chen,
- Xuke Lu,
- Delong Wang,
- Junjuan Wang,
- Shuai Wang,
- Lixue Guo,
- Quanjia Chen,
- Wuwei Ye,
- Wuwei Ye
Affiliations
- Wenhua Chen
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Wenhua Chen
- Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Urumqi, China
- Yupeng Cui
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Yunxin He
- Hunan Institute of Cotton Science, Changde, Hunan, China
- Lanjie Zhao
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Ruifeng Cui
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Xiaoyu Liu
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Hui Huang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Yuexin Zhang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Yapeng Fan
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Xixian Feng
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Kesong Ni
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Tiantian Jiang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Mingge Han
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Yuqian Lei
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Mengyue Liu
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Yuan Meng
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Xiugui Chen
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Xuke Lu
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Delong Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Junjuan Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Shuai Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Lixue Guo
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Quanjia Chen
- Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Urumqi, China
- Wuwei Ye
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences/Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, Henan, China
- Wuwei Ye
- Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Urumqi, China
- DOI
- https://doi.org/10.3389/fpls.2023.1246677
- Journal volume & issue
-
Vol. 14
Abstract
A-galactosidases (AGALs), the oligosaccharide (RFO) catabolic genes of the raffinose family, play crucial roles in plant growth and development and in adversity stress. They can break down the non-reducing terminal galactose residues of glycolipids and sugar chains. In this study, the whole genome of AGALs was analyzed. Bioinformatics analysis was conducted to analyze members of the AGAL family in Gossypium hirsutum, Gossypium arboreum, Gossypium barbadense, and Gossypium raimondii. Meanwhile, RT-qPCR was carried out to analyze the expression patterns of AGAL family members in different tissues of terrestrial cotton. It was found that a series of environmental factors stimulated the expression of the GhAGAL3 gene. The function of GhAGAL3 was verified through virus-induced gene silencing (VIGS). As a result, GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton, indicating that GhAGAL3 responded to NaCl stress. The increase in raffinose content improved the tolerance of cotton. Findings in this study lay an important foundation for further research on the role of the GhAGAL3 gene family in the molecular mechanism of abiotic stress resistance in cotton.
Keywords
- α-galactosidase (AGALs)
- Gossypium hirsutum
- raffinose family oligosaccharides (RFOs)
- abiotic stresses
- functional verification
