Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of <i>Burkholderia</i>

Abstract

The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species.

Keywords

• <i>burkholderia</i>
• β-lactamases
• molecular typing
• polymerase chain reaction