Ultrasound-assisted extraction of Cordyceps cicadae polyphenols: Optimization, LC-MS characterization, antioxidant and DNA damage protection activity evaluation

Zizhong Tang; Wenjie Lin; Jing Yang; Shiling Feng; Yihan Qin; Yirong Xiao; Hong Chen; Yuntao Liu; Hui Chen; Tongliang Bu; Qinfeng Li; Huipeng Yao; Chunbang Ding

Arabian Journal of Chemistry (Aug 2022)

Ultrasound-assisted extraction of Cordyceps cicadae polyphenols: Optimization, LC-MS characterization, antioxidant and DNA damage protection activity evaluation

Zizhong Tang,
Wenjie Lin,
Jing Yang,
Shiling Feng,
Yihan Qin,
Yirong Xiao,
Hong Chen,
Yuntao Liu,
Hui Chen,
Tongliang Bu,
Qinfeng Li,
Huipeng Yao,
Chunbang Ding

Affiliations

Zizhong Tang: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China; Corresponding authors at: Xinkang road, Ya’an, Sichuan 625014, China.
Wenjie Lin: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Jing Yang: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Shiling Feng: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Yihan Qin: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Yirong Xiao: Sichuan Agricultural University Hospital, Ya’an 625014, China
Hong Chen: College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Yuntao Liu: College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
Hui Chen: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China; Corresponding authors at: Xinkang road, Ya’an, Sichuan 625014, China.
Tongliang Bu: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Qinfeng Li: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Huipeng Yao: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China
Chunbang Ding: College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China

Journal volume & issue: Vol. 15, no. 8
p. 103953

Abstract

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In this study, ultrasound-assisted extraction of polyphenols from C. cicadae was optimized by response surface methodology (RSM). The optimized conditions were determined as extraction time of 39 min, liquid-to-solid ratio of 1:29 g/mL, extraction temperature of 69 °C and ethanol concentration of 55% with a yield of 21.9 mg gallic acid equivalent/g dry weight. Four resins were used for polyphenol purification. D101 resin had the highest ratio of adsorption and was further applied in polyphenol purification test. A total of 19 different phenolic compounds were identified by LC-MS, including 12 phenolic acids and 7 organic acids. In addition, C. cicadae polyphenols displayed higher antioxidant activity in vitro and anti-aging activity of C. elegans in vivo. Lastly, C. cicadae polyphenols showed the potential to protect DNA from oxidative damage. Overall, our results suggest that polyphenols from C. cicadae may be considered as novel sources of anti-oxidation, anti-aging and recommended as reagents to protect DNA from oxidative damage in food and pharmaceutical industries.

Published in Arabian Journal of Chemistry

ISSN: 1878-5352 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Chemistry
Website: http://www.journals.elsevier.com/arabian-journal-of-chemistry/

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