Scientific Reports (Jun 2024)

Increased expression of miR-320b in blood plasma of patients in response to SARS-CoV-2 infection

  • Aline de Souza Nicoletti,
  • Marília Berlofa Visacri,
  • Carla Regina da Silva Correa da Ronda,
  • Julia Tiemi Siguemoto,
  • Carolini Motta Neri,
  • Rafael Nogueira de Souza,
  • Deise de Souza Ventura,
  • Adriana Eguti,
  • Lilian Ferreira de Souza Silva,
  • Mauricio Wesley Perroud Junior,
  • Keini Buosi,
  • Mehrsa Jalalizadeh,
  • Franciele Dionato,
  • Luciana Dal Col,
  • Cristiane Giacomelli,
  • Patrícia Leme,
  • Leonardo Oliveira Reis,
  • Luiz Augusto dos Santos,
  • Nelson Durán,
  • Wagner José Fávaro,
  • José Luiz da Costa,
  • Carolina Dagli-Hernandez,
  • Patricia Moriel,
  • Eder de Carvalho Pincinato

DOI
https://doi.org/10.1038/s41598-024-64325-9
Journal volume & issue
Vol. 14, no. 1
pp. 1 – 10

Abstract

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Abstract Coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Recent research has demonstrated how epigenetic mechanisms regulate the host–virus interactions in COVID-19. It has also shown that microRNAs (miRNAs) are one of the three fundamental mechanisms of the epigenetic regulation of gene expression and play an important role in viral infections. A pilot study published by our research group identified, through next-generation sequencing (NGS), that miR-4433b-5p, miR-320b, and miR-16–2-3p are differentially expressed between patients with COVID-19 and controls. Thus, the objectives of this study were to validate the expression of these miRNAs using quantitative real-time polymerase chain reaction (qRT-PCR) and to perform in silico analyses. Patients with COVID-19 (n = 90) and healthy volunteers (n = 40) were recruited. MiRNAs were extracted from plasma samples and validated using qRT-PCR. In addition, in silico analyses were performed using mirPath v.3 software. MiR-320b was the only miRNA upregulated in the case group com-pared to the control group. The in silico analyses indicated the role of miR-320b in the regulation of the KITLG gene and consequently in the inflammatory process. This study confirmed that miR-320b can distinguish patients with COVID-19 from control participants; however, further research is needed to determine whether this miRNA can be used as a target or a biomarker.

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