Frontiers in Cell and Developmental Biology (Jun 2021)

Establishment and Characterization of a HER2-Positive Cell Line Derived From the Pleural Effusion of a Drug-Resistant Breast Cancer Patient

  • Zhaoqing Li,
  • Zhaoqing Li,
  • Zhaoqing Li,
  • Wenying Zhuo,
  • Wenying Zhuo,
  • Wenying Zhuo,
  • Lini Chen,
  • Lini Chen,
  • Xun Zhang,
  • Xun Zhang,
  • Cong Chen,
  • Cong Chen,
  • Dengdi Hu,
  • Dengdi Hu,
  • Dengdi Hu,
  • Yongxia Chen,
  • Yongxia Chen,
  • Jingjing Yang,
  • Jingjing Yang,
  • Yulu Zhou,
  • Yulu Zhou,
  • Misha Mao,
  • Misha Mao,
  • Ling Xu,
  • Ling Xu,
  • Siwei Ju,
  • Siwei Ju,
  • Jun Shen,
  • Jun Shen,
  • Qinchuan Wang,
  • Qinchuan Wang,
  • Minjun Dong,
  • Minjun Dong,
  • Shuduo Xie,
  • Shuduo Xie,
  • Jichun Zhou,
  • Jichun Zhou,
  • Linbo Wang,
  • Linbo Wang

DOI
https://doi.org/10.3389/fcell.2021.680968
Journal volume & issue
Vol. 9

Abstract

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Drug resistance is a daunting challenge in the treatment of breast cancer, making it an urgent problem to solve in studies. Cell lines are important tools in basic and preclinical studies; however, few breast cell lines from drug-resistant patients are available. Herein, we established a novel HER2-positive breast cancer cell line from the pleural effusion of a drug-resistant metastatic breast cancer patient. This cell line has potent proliferative capability and tumorigenicity in nude mice but weak invasive and colony-forming capability. The molecular subtype of the cell line and its sensitivity to chemotherapeutics and HER2-targeting agents are different from those of its origin, suggesting that the phenotype changes between the primary and metastatic forms of breast cancer.

Keywords