Association between Maternal Non-Coding Interferon-λ Polymorphisms and Congenital Zika Syndrome in a Cohort from Brazilian Northeast
Átila Duque Rossi,
Fabio Rueda Faucz,
Adriana Melo,
Girlene Souza de Azevedo,
Paula Pezzuto,
Ohanna Cavalcanti de Lima Bezerra,
Fernanda Saloum de Neves Manta,
Tamiris Azamor,
Bruno Luiz Fonseca Schamber-Reis,
Amilcar Tanuri,
Milton Ozório Moraes,
Renato Santana Aguiar,
Constantine A. Stratakis,
Cynthia Chester Cardoso
Affiliations
Átila Duque Rossi
Laboratório de Virologia Molecular, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Fabio Rueda Faucz
Section on Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20814, USA
Adriana Melo
Instituto de Pesquisa Professor Joaquim Amorim Neto (IPESQ), Campina Grande 58406-115, Brazil
Girlene Souza de Azevedo
Instituto de Pesquisa Professor Joaquim Amorim Neto (IPESQ), Campina Grande 58406-115, Brazil
Paula Pezzuto
Laboratório de Virologia Molecular, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Ohanna Cavalcanti de Lima Bezerra
Laboratório de Hanseníase, Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro 21040-900, Brazil
Fernanda Saloum de Neves Manta
Laboratório de Hanseníase, Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro 21040-900, Brazil
Tamiris Azamor
Laboratório de Hanseníase, Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro 21040-900, Brazil
Bruno Luiz Fonseca Schamber-Reis
Faculdade de Ciências Médicas de Campina Grande, Núcleo de Genética Médica, Centro Universitário UniFacisa, Campina Grande 58408-326, Brazil
Amilcar Tanuri
Laboratório de Virologia Molecular, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Milton Ozório Moraes
Laboratório de Hanseníase, Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro 21040-900, Brazil
Renato Santana Aguiar
Laboratório de Virologia Molecular, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Constantine A. Stratakis
Section on Endocrinology and Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20814, USA
Cynthia Chester Cardoso
Laboratório de Virologia Molecular, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Congenital Zika syndrome (CZS) is characterized by a diverse group of congenital malformations induced by ZIKV infection during pregnancy. Type III interferons have been associated with placental immunity against ZIKV and restriction of vertical transmission in mice, and non-coding single-nucleotide polymorphisms (SNPs) on these genes are well known to influence susceptibility to other viral infections. However, their effect on ZIKV pathogenesis has not yet been explored. To investigate whether maternal non-coding SNPs at IFNL genes are associated with CZS, 52 women infected with ZIKV during pregnancy were enrolled in a case–control association study. A total of 28 women were classified as cases and 24 as controls based on the presence or absence of CZS in their infants, and seven Interferon-λ non-coding SNPs (rs12980275, rs8099917, rs4803217, rs4803219, rs8119886, rs368234815, rs12979860) were genotyped. The results of logistic regression analyses show an association between the G allele at rs8099917 and increased susceptibility to CZS under a log-additive model (adjustedOR = 2.80; 95%CI = 1.14–6.91; p = 0.02), after adjustment for trimester of infection and genetic ancestry. These results provide evidence of an association between Interferon-λ SNPs and CZS, suggesting rs8099917 as a promising candidate for further studies on larger cohorts.
