Effects of sphingosine-1-phosphate on gene expression of two cell mouse embryos induced by C2-Ceramide

Abstract

Objective: As to understand the sphingosine-1-phosphate (S1P) on gene expression of the pre-implantation mouse embryo induced by C2-Ceramide (CED). Materials and methods: Global gene-expression profiling of two cell mouse embryo was performed using Affymetrix Gene Chip® Mouse Genome 430 2.0 Array. Results: Fifty-five genes were identified with significant expression changes that are functionally involved in the two cell embryo after treated by S1P and CED. Of these genes, 30 were up-regulated and 25 were down-regulated. Genes Atm, Cdkn1b and Fgrfr2 are identified to be involved in the protective role of S1P against the apoptotic signals induced by CED. Conclusions: This study provides a map of genes in the pre-implantation two cell mouse embryo. Further investigation based on these data will provide a better understanding of the effects of S1P on the pre-implantation embryos in other mammalian species, especially human.

Keywords

• Pre-implantation mouse embryos
• Two cell embryo
• Gene expression
• Sphingosine-1-phosphate
• Ceramide