Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli

Mathew Stracy; Marcin Jaciuk; Stephan Uphoff; Achillefs N. Kapanidis; Marcin Nowotny; David J. Sherratt; Pawel Zawadzki

doi:10.1038/ncomms12568

Nature Communications (Aug 2016)

Single-molecule imaging of UvrA and UvrB recruitment to DNA lesions in living Escherichia coli

Mathew Stracy,
Marcin Jaciuk,
Stephan Uphoff,
Achillefs N. Kapanidis,
Marcin Nowotny,
David J. Sherratt,
Pawel Zawadzki

Affiliations

Mathew Stracy: Department of Biochemistry, University of Oxford
Marcin Jaciuk: Laboratory of Protein Structure, International Institute of Molecular and Cell Biology
Stephan Uphoff: Department of Biochemistry, University of Oxford
Achillefs N. Kapanidis: Department of Physics, Biological Physics Research Group, Clarendon Laboratory, University of Oxford
Marcin Nowotny: Laboratory of Protein Structure, International Institute of Molecular and Cell Biology
David J. Sherratt: Department of Biochemistry, University of Oxford
Pawel Zawadzki: Department of Biochemistry, University of Oxford

DOI: https://doi.org/10.1038/ncomms12568
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 9

Abstract

Read online

Nucleotide excision repair is able to identify and remove a wide range of DNA helix distorting lesions from the genome. Here the authors use single molecule imaging of UvrA and UvrB molecules and suggest a two-step ‘scan and recruit’ model for UvrA function.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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