Analysis of fecal microbiome and metabolome changes in goats with pregnant toxemia

Bingyan Jin; Ruoqian Wang; Jiada Hu; Yan Wang; Panpan Cheng; Jiancong Zhang; Jiahui Zhang; Gang Xue; Yan Zhu; Yunhai Zhang; Fugui Fang; Ya Liu; Yunsheng Li

doi:10.1186/s12917-023-03849-0

BMC Veterinary Research (Jan 2024)

Analysis of fecal microbiome and metabolome changes in goats with pregnant toxemia

Bingyan Jin,
Ruoqian Wang,
Jiada Hu,
Yan Wang,
Panpan Cheng,
Jiancong Zhang,
Jiahui Zhang,
Gang Xue,
Yan Zhu,
Yunhai Zhang,
Fugui Fang,
Ya Liu,
Yunsheng Li

Affiliations

Bingyan Jin: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Ruoqian Wang: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Jiada Hu: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Yan Wang: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Panpan Cheng: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Jiancong Zhang: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Jiahui Zhang: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Gang Xue: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Yan Zhu: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Yunhai Zhang: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Fugui Fang: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Ya Liu: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University
Yunsheng Li: Anhui Provincial Key Laboratory of Local Livestock Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agriculture University

DOI: https://doi.org/10.1186/s12917-023-03849-0
Journal volume & issue: Vol. 20, no. 1
pp. 1 – 14

Abstract

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Abstract Background Pregnancy toxemia is a common disease, which occurs in older does that are pregnant with multiple lambs in the third trimester. Most of the sick goats die within a few days, which can seriously impact the economic benefits of goat breeding enterprises. The disease is believed to be caused by malnutrition, stress, and other factors, that lead to the disorder of lipid metabolism, resulting in increased ketone content, ketosis, ketonuria, and neurological symptoms. However, the changes in gut microbes and their metabolism in this disease are still unclear. The objective of this experiment was to evaluate the effect of toxemia of pregnancy on the fecal microbiome and metabolomics of does. Results Eight pregnant does suspected of having toxemia of pregnancy (PT group) and eight healthy does during the same pregnancy (NC group) were selected. Clinical symptoms and pathological changes at necropsy were observed, and liver tissue samples were collected for pathological sections. Jugular venous blood was collected before morning feeding to detect biochemical indexes. Autopsy revealed that the liver of the pregnancy toxemia goat was enlarged and earthy yellow, and the biochemical results showed that the serum levels of aspartate aminotransferase (AST) and β-hydroxybutyric acid (B-HB) in the PT group were significantly increased, while calcium (Ca) levels were significantly reduced. Sections showed extensive vacuoles in liver tissue sections. The microbiome analysis found that the richness and diversity of the PT microbiota were significantly reduced. Metabolomic analysis showed that 125 differential metabolites were screened in positive ion mode and enriched in 12 metabolic pathways. In negative ion mode, 100 differential metabolites were screened and enriched in 7 metabolic pathways. Conclusions Evidence has shown that the occurrence of pregnancy toxemia is related to gut microbiota, and further studies are needed to investigate its pathogenesis and provide research basis for future preventive measures of this disease.

Published in BMC Veterinary Research

ISSN: 1746-6148 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: http://bmcvetres.biomedcentral.com

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