DNA Replication Through Strand Displacement During Lagging Strand DNA Synthesis in <i>Saccharomyces cerevisiae</i>

Abstract

This review discusses a set of experimental results that support the existence of extended strand displacement events during budding yeast lagging strand DNA synthesis. Starting from introducing the mechanisms and factors involved in leading and lagging strand DNA synthesis and some aspects of the architecture of the eukaryotic replisome, we discuss studies on bacterial, bacteriophage and viral DNA polymerases with potent strand displacement activities. We describe proposed pathways of Okazaki fragment processing via short and long flaps, with a focus on experimental results obtained in Saccharomyces cerevisiae that suggest the existence of frequent and extended strand displacement events during eukaryotic lagging strand DNA synthesis, and comment on their implications for genome integrity.

Keywords

• DNA replication
• lagging strand DNA synthesis
• Okazaki fragment processing
• strand displacement DNA synthesis
• DNA helicases
• flap endonucleases