International Journal of Molecular Sciences (Nov 2013)

Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation

  • Qiang Liu,
  • Bing Wang,
  • Takanori Katsube,
  • Sai Jun Fan,
  • Fei-Yue Fan,
  • Hui Zhao,
  • Xu Su,
  • Jian Xiang Liu,
  • Jia Cao,
  • Li Qing Du,
  • Chang Xu,
  • Yan Wang

DOI
https://doi.org/10.3390/ijms141122449
Journal volume & issue
Vol. 14, no. 11
pp. 22449 – 22461

Abstract

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Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.

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